|
| Status |
Public on May 28, 2009 |
| Title |
MCF-7 Luciferase Replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
MCF-7 Cells Luciferase Knockdown (control)
|
| Organism |
Homo sapiens |
| Characteristics |
MCF-7 Cells Luciferase Knockdown (control)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared using Trizol Reagent according to the manufacturer’s instructions (Invitrogen) and was further purified using RNeasy columns (Qiagen).
|
| Label |
Biotin
|
| Label protocol |
Seven μg of total RNA were subjected to One-Cycle Target Labeling Assay (Affymetrix) to generate biotinylated cRNA targets for hybridization to Affymetrix U133A 2.0 microarray.
|
| |
|
| Hybridization protocol |
Standard hybridization to Affymetrix U133A 2.0 microarray.
|
| Scan protocol |
Standard scanning protocol using GeneChip Scanner.
|
| Description |
N/A
|
| Data processing |
The raw data were processed by Affymetrix GCOS software to obtain detection calls and signal values, and then normalized by scaling. Signals were then used in further analysis, namely batch effect adjusted and log2 median-normalized using COMBAT R.
|
| |
|
| Submission date |
Sep 28, 2008 |
| Last update date |
May 28, 2009 |
| Contact name |
W. Lee Kraus |
| E-mail(s) |
lee.kraus@utsouthwestern.edu
|
| Organization name |
UT Southwestern Medical Center
|
| Street address |
5323 Harry Hines Blvd.
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390-8511 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (4)
|
| GSE12952 |
Expression Analysis Upon PARP-1 or PARG Knockdown in MCF-7 Cells |
| GSE12971 |
MCF-7 Luciferase, PARP-1, PARG, SIRT1, and macroH2A Knockdown |
| GSE13459 |
Expression analysis upon SIRT1 knockdown in MCF-7 cells |
| GSE13577 |
SIRT1-Dependent Gene Regulation Through Promoter-Directed Recruitment of a Nuclear NAD+ Synthase |
|
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