|
| Status |
Public on Jul 23, 2024 |
| Title |
EG_2is_1 |
| Sample type |
RNA |
| |
|
| Source name |
Embryonic germ cells, 2i
|
| Organism |
Mus musculus |
| Characteristics |
Sex: Male
batch: 1
|
| Growth protocol |
Serum culture conditions contain 15% FCS, 0.1 mM MEM nonessential amino acids, 2 mM l-glutamine, 1 mM sodium pyruvate, 0.1 mM 2-mercaptoethanol and 1000 U/mL LIF in DMEM-F12 with maintenance on a mouse embryonic fibroblast (MEF) feeder layer. 2i culture conditions contain 1 μM PD0325901, 3 μM CHIR99021 and 1000 U/mL LIF in N2B27 medium, maintained on laminin (10 μg/ml). Cells were incubated at 5% CO2, 95% relative humidity.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA (100 ng) was isolated using the Qiagen RNeasy Mini kit.
|
| Label |
Biotin
|
| Label protocol |
100 ng of total RNA was processed using the Ambion WT Expression kit and Affymetrix GeneChip WT Terminal Labelling and Hybridisation kit.
|
| |
|
| Hybridization protocol |
100 ng of total RNA was processed using the Ambion WT Expression kit and Affymetrix GeneChip WT Terminal Labelling and Hybridisation kit.
|
| Scan protocol |
The GeneChip scanner was used to scan all micorarrays.
|
| Data processing |
Data was preprocessed in R using robust multiarray averaging, variance stabilising transformation and Combat batch correction.
|
| |
|
| Submission date |
Jul 03, 2018 |
| Last update date |
Jul 23, 2024 |
| Contact name |
Kirsten R McEwen |
| E-mail(s) |
kirsten.mcewen@imperial.ac.uk
|
| Organization name |
Imperial College London
|
| Department |
MRC Clinical Sciences Centre
|
| Lab |
Reprogramming and Chromatin
|
| Street address |
Du Cane Road
|
| City |
London |
| ZIP/Postal code |
W12 0NN |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL17777 |
| Series (1) |
| GSE116565 |
Signalling pathways drive heterogeneity of ground state pluripotency |
|
