|
| Status |
Public on Oct 20, 2008 |
| Title |
HeLa_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
HeLa
|
| Organism |
Homo sapiens |
| Characteristics |
HeLa
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction was performed using a Qiagen RNeasy mini kit following the manufacturer's directions.
|
| Label |
biotin
|
| Label protocol |
Labeling was performed using Affymetrix's IVT labeling kit following manufacturer's directions.
|
| |
|
| Hybridization protocol |
20 micrograms of biotin labeled RNA was fragmented to ~200 bp fragments by incubating in a buffer containing 200 mM Tris-Acetate pH 8.2, 500 mM Potassium Acetate and 500 Magnesium Acetate for 35 minutes at 95C. Fragments were hybridized to Affymetrix U133A plus 2.0 chips for 16 hrs, washed and stained on an Affymetrix fluidics station.
|
| Scan protocol |
Scanning was performed with the Affymetrix GeneChip scanner using default settings.
|
| Description |
None
|
| Data processing |
Raw signal values were normalized using RMA.
|
| |
|
| Submission date |
Sep 22, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Raja Jothi |
| E-mail(s) |
jothi@mail.nih.gov
|
| Organization name |
National Institutes of Health
|
| Department |
National Institute of Environmental Health Sciences
|
| Lab |
Systems Biology
|
| Street address |
111 TW Alexander Drive; A314
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE12889 |
CTCF demarcates chromatin domains |
|
| Relations |
| Reanalyzed by |
GSE119087 |
