|
| Status |
Public on Dec 04, 2008 |
| Title |
DND41_CompE_72hs |
| Sample type |
RNA |
| |
|
| Source name |
DND41 cell line
|
| Organism |
Homo sapiens |
| Characteristics |
DND41 T-ALL cell line
|
| Treatment protocol |
Cells were treated with a gamma-secretase inhibitor, CompE 100nM, for 72 hs
|
| Growth protocol |
Cells were cultured in RPMI 1640 media with 10% FBS, supplemented with pen/strep
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Ambion RNAqueous miniquit following standard procedures
|
| Label |
biotin
|
| Label protocol |
Affymetrix standard procedures
|
| |
|
| Hybridization protocol |
Affymetrix standard procedures
|
| Scan protocol |
Affymetrix standard procedures
|
| Description |
This sample was used as experimental sample in an experiment testing the effects of inhibition of NOTCH1 signaling using a gamma-secretase inhibitor (CompE)
|
| Data processing |
MAS5.0
|
| |
|
| Submission date |
Sep 19, 2008 |
| Last update date |
Dec 04, 2008 |
| Contact name |
Adolfo A Ferrando |
| E-mail(s) |
af2196@columbia.edu
|
| Phone |
212-851-4611
|
| Organization name |
Columbia University
|
| Department |
Institute for Cancer Genetics
|
| Street address |
1130 St Nicholas Ave Irving Cancer Research Center 5-505A
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE12868 |
ChIP-on-chip significance analysis reveals large-scale binding and regulation by human transcription factor oncogenes |
|
