|
| Status |
Public on Jun 06, 2018 |
| Title |
OGT_SCKO 3 |
| Sample type |
RNA |
| |
|
| Source name |
OGT_SCKO 3
|
| Organism |
Mus musculus |
| Characteristics |
tissue: sciatic nerves
age: 1month
strain: C56 BL6
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from sciatic nerves of control and OGT-KO mice using Trizol (Invitrogen).Both control and KO mice are on a C56 BL6 background.
|
| Label |
Cy5
|
| Label protocol |
cDNAs were chemically labeled with Kreatech ULS RNA labeling kit (Kreatech Diagnostics). Per reaction, 3ug of each RNA (+water=16ul) was mixed with Kreatech 10x labeling buffer (2ul) and Kreatech cy5/DY-ULS (2ul). The reactions were incubated at 85C for 15 minutes in the dark and placed on ice for 3 minutes. Labeled cDNA was purified with Quaigen PCR purification columns according to manufacturer’s protocol. cDNAs were quantitated on a Nanodrop spectrophotometer
|
| |
|
| Hybridization protocol |
The balanced aRNAs were suspended in Agilent 2X Gene Expression buffer (55ul), Agilent 10X Blocking agent (11ul), and Kreablock (6ul). The hybridization solutions were applied to Agilent Mouse v2 4x44K microarrays (G4846A-026655). Hybridization was carried out at 65C for 20 hours. Washing procedures were carried out according to Agilent gene expression protocols
|
| Scan protocol |
Slides were scanned on an Agilent C-class Microarray scanner to detect Cy5 fluorescence, according to manufacturer's specifications. Gridding and analysis of images was performed using Feature Extraction (v11.5.1.1, Agilent Technologies)
|
| Description |
gene expression
|
| Data processing |
Gridding and analysis of images was performed using Feature Extraction (v11.5.1.1, Agilent Technologies). Raw data was quantile normalized and log2 signal intensities reported.
|
| |
|
| Submission date |
Jun 05, 2018 |
| Last update date |
Jun 06, 2018 |
| Contact name |
sungsu kim |
| E-mail(s) |
sungsukim@wustl.edu
|
| Organization name |
washington university
|
| Street address |
600 S. euclid ave
|
| City |
st. Louis |
| ZIP/Postal code |
MO |
| Country |
USA |
| |
|
| Platform ID |
GPL11202 |
| Series (1) |
| GSE115333 |
Jun O-GlcNAcylatSchwann cell O-GlcNAcylation promotes peripheral nerve remyelination via attenuation of the AP-1 transcription factor JUNion promotes peripheral nerve remyelination after nerve injury |
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