|
| Status |
Public on Apr 30, 2019 |
| Title |
H1975_TKI_b |
| Sample type |
SRA |
| |
|
| Source name |
H1975 Cell line
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: WZ4002
dose: 300nM
cell line: H1975
|
| Treatment protocol |
Cells were treated with vehicle or the indicated concentrations of Gefitinib/WZ4002 for 2-3 weeks
|
| Growth protocol |
cells were grown in RPMI supplemented with 10% FBS at 37 degrees C and 5% CO2
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total cellular RNA using Qiagen RNEasy kit
Strand-specific RNA-seq library preparation using the Ovation Universal RNA-Seq System 1-96; For PC9 and MGH119 - Illumina Poly-A kit followed by NEB's Ultra Directional Library Prep
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
RNA-seq reads were aligned to the hg19genome assembly using STAR with default parameter
Counted with htseq-0.6.1 based on UCSC gene annotations
Genome_build: hg19
Supplementary_files_format_and_content: read count abundance measurements were generated using htseq
|
| |
|
| Submission date |
May 18, 2018 |
| Last update date |
Apr 30, 2019 |
| Contact name |
Sana Raoof |
| E-mail(s) |
sanaraoofmgh@gmail.com
|
| Organization name |
Massachusetts General Hospital
|
| Street address |
175 Cambridge St
|
| City |
Boston |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
|
| Platform ID |
GPL16791 |
| Series (1) |
| GSE114647 |
Targeting FGFR overcomes EMT-mediated resistance in EGFR mutant non-small cell lung cancer |
|
| Relations |
| BioSample |
SAMN09223853 |
| SRA |
SRX4099673 |
