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Sample GSM3146123 Query DataSets for GSM3146123
Status Public on May 19, 2018
Title BJAB-KSHV rep2
Sample type SRA
 
Source name BJAB-KSHV
Organism Homo sapiens
Characteristics cell line: BJAB-KSHV
cell type: B-cell line
Treatment protocol 100µM Cobalt chloride for 48 hours
Growth protocol Cells were grown in RPMI medium supplemented with 7% bovine growth serum at 37°C/5%CO2
Extracted molecule total RNA
Extraction protocol RNA from cells growing in culture was isolated using trizole reagent by standard phenol chloroform extraction
Library for RNA sequencing was preapred using Illumina RNA sequencing sample prep kit
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Data processing CLC bio (Qiagen) was used for read alignment
The reads for sequencing data were aligned with HHV8 or human genome
Differential gene expression were calculated CLC bio software
Genome_build: hg19, NC_009333.1
 
Submission date May 18, 2018
Last update date May 19, 2018
Contact name Rajnish Kumar Singh
E-mail(s) rajsingh@upenn.edu
Phone 2157460116
Organization name University of Pennsylvania
Department Otorhinolaryngology
Street address 202B Johnson Pavilion 3610 Hamilton walk
City Philadelphia
State/province Pennsylvania
ZIP/Postal code 19104
Country USA
 
Platform ID GPL11154
Series (1)
GSE114625 Metabolic reprogramming of Kaposi’s sarcoma associated herpes virus infected B-cells in hypoxia
Relations
BioSample SAMN09223249
SRA SRX4099077

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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