|
Status |
Public on May 19, 2018 |
Title |
BJAB-KSHV rep2 |
Sample type |
SRA |
|
|
Source name |
BJAB-KSHV
|
Organism |
Homo sapiens |
Characteristics |
cell line: BJAB-KSHV cell type: B-cell line
|
Treatment protocol |
100µM Cobalt chloride for 48 hours
|
Growth protocol |
Cells were grown in RPMI medium supplemented with 7% bovine growth serum at 37°C/5%CO2
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA from cells growing in culture was isolated using trizole reagent by standard phenol chloroform extraction Library for RNA sequencing was preapred using Illumina RNA sequencing sample prep kit
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2000 |
|
|
Data processing |
CLC bio (Qiagen) was used for read alignment The reads for sequencing data were aligned with HHV8 or human genome Differential gene expression were calculated CLC bio software Genome_build: hg19, NC_009333.1
|
|
|
Submission date |
May 18, 2018 |
Last update date |
May 19, 2018 |
Contact name |
Rajnish Kumar Singh |
E-mail(s) |
rajsingh@upenn.edu
|
Phone |
2157460116
|
Organization name |
University of Pennsylvania
|
Department |
Otorhinolaryngology
|
Street address |
202B Johnson Pavilion 3610 Hamilton walk
|
City |
Philadelphia |
State/province |
Pennsylvania |
ZIP/Postal code |
19104 |
Country |
USA |
|
|
Platform ID |
GPL11154 |
Series (1) |
GSE114625 |
Metabolic reprogramming of Kaposi’s sarcoma associated herpes virus infected B-cells in hypoxia |
|
Relations |
BioSample |
SAMN09223249 |
SRA |
SRX4099077 |