|
| Status |
Public on May 15, 2018 |
| Title |
SCP2 |
| Sample type |
RNA |
| |
|
| Source name |
Rat Schwann cell precursors
|
| Organism |
Rattus norvegicus |
| Characteristics |
cell type: Rat Schwann cell precursors
culture conditions: Normal
|
| Treatment protocol |
Rat oligodendrocyte precursors are cultured in modified Sato medium for around one week.
|
| Growth protocol |
Rat Schwann cell precursors are cultured in modified Sato medium and grown.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA samples were extracted from cells using ISOGEN（NIPPON GENE) and RNeasy mini kit (QIAGEN).
|
| Label |
Cy5
|
| Label protocol |
Amino Allyl aRNA was synthesis by Amino Allyl MessageAmp II aRNA Amplification Kit (Ambion#1753). CyeDye Coupling and fragmentation were performed as the protocol supplied by TORAY Industries, Inc..
|
| |
|
| Hybridization protocol |
Hybridized for 16 h at 37 C with rotary shake (250 rpm). Hybridization buffer and washing protocol was followed by the protocol supplied by TORAY Industries, Inc..
|
| Scan protocol |
3D-Gene Scanner ((Toray Industries Inc., Tokyo, Japan) was used for scanning. Images were quantified using Extraction(Toray Industries Inc., Tokyo, Japan).
|
| Description |
Rat Schwann cells
|
| Data processing |
The raw data of each spot was normalized by substitution with a mean intensity of the background signal determined by all blank spots’ signal intensities of 95% confidence intervals.
|
| |
|
| Submission date |
May 14, 2018 |
| Last update date |
May 15, 2018 |
| Contact name |
Satoshi Kondo |
| Organization name |
Toray Industries,Inc.
|
| Street address |
10-1 tebiro 6-chome
|
| City |
Kamakura |
| State/province |
Kanagawa |
| ZIP/Postal code |
248-8555 |
| Country |
Japan |
| |
|
| Platform ID |
GPL22440 |
| Series (1) |
| GSE114441 |
Schwann cell precursor mRNA in normal culture conditions |
|
