|
| Status |
Public on Jun 12, 2018 |
| Title |
S164.G3 |
| Sample type |
SRA |
| |
|
| Source name |
human_iPS-derived cerebral organoid
|
| Organism |
Homo sapiens |
| Characteristics |
donor id: H3
clone id: YH10
age (weeks): 5.57
date captured: 2016-05-04
Sex: Male
cell type: iPS-derived cerebral organoid
organoid id: H3.2
capture plate: 1784054172
capture chamber: 1784054172.G3
|
| Growth protocol |
Cerebral organoids from Kadoshima et al., 2013
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Fluidigm C1 small IFC (100-5759; 100-6201); SMARTer® Ultra™ Low RNA Kit (634832)
Nextera XT tagmentation kit (FC-131-096)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Trim Galore 3.7
HISAT2
subread-1.5.0 function featureCounts
GRCh38.79.gtf
Genome_build: GRCh38.p2/Clint_PTRv2; UCSC: panTro6; GCF_002880755.1
Supplementary_files_format_and_content: log(CPM+1)
|
| |
|
| Submission date |
May 10, 2018 |
| Last update date |
Jun 12, 2018 |
| Contact name |
Alex Aaron Pollen |
| E-mail(s) |
alex.pollen@ucsf.edu
|
| Organization name |
UCSF
|
| Department |
Neurology
|
| Lab |
Pollen
|
| Street address |
35 Medical Center Way
|
| City |
San Francisco |
| State/province |
California |
| ZIP/Postal code |
94143-0525 |
| Country |
USA |
| |
|
| Platform ID |
GPL16791 |
| Series (1) |
| GSE113931 |
High-resolution comparative analysis of great ape genomes |
|
| Relations |
| BioSample |
SAMN09197177 |
| SRA |
SRX4071488 |
