|
| Status |
Public on Jun 20, 2018 |
| Title |
MBC05 |
| Sample type |
RNA |
| |
|
| Source name |
Breast
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: medullary breast carcinoma
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions. A cleanup with RNAII Nucleospin was conducted according Macherey Nagel recommendations
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the Affymetrix two round amplification protocol from 100ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of labelled cRNA were hybridized for 16 hr on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station FS450.
|
| Scan protocol |
GeneChips were scanned using Affymetrix GeneChip GCS3000 scanner.
|
| Data processing |
Raw data were controlled using Genechip Operating Software and Affymetrix MAS5 algorithm to control the quality of hybridization and synthesis steps. Raw data were analysed using R/Bioconductor and normalized by RMA method.
|
| |
|
| Submission date |
May 08, 2018 |
| Last update date |
Jun 20, 2018 |
| Contact name |
Cecile Laurent |
| E-mail(s) |
cecile.laurent@curie.fr
|
| Organization name |
Institut Curie
|
| Street address |
26 rue d'ulm
|
| City |
Paris Cedex 05 |
| ZIP/Postal code |
75248 |
| Country |
France |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE114168 |
Medullary breast carcinoma, a triple-negative breast cancer subtype associated with BCLG overexpression. |
| GSE114269 |
Medullary breast carcinoma, a triple-negative breast cancer subtype associated with BCLG overexpression and BRCAness |
|
