|
| Status |
Public on Jun 29, 2018 |
| Title |
human iPSC which is used to induce cancer stem cells |
| Sample type |
RNA |
| |
|
| Source name |
original human iPSC 201B7
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
age: 36
|
| Treatment protocol |
Human iPS cells obtained from Riken.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Using the RNeasy column purification (QIAGEN) total RNA was prepared.
|
| Label |
Cy3
|
| Label protocol |
Cyanine-3 (Cy3) labeled cRNA was prepared from 100 ng RNA using Low Input Quick Amp Labeling Kit, One-Color (Agilent Technologies) according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
600 ng of Cy3-labelled cRNA was fragmented at 60°C for 30 minutes and hybridized for 17 hours at 65°C with Gene Expression Hybridization Kit (Agilent Technologies).
|
| Scan protocol |
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2565CA) using one color scan setting for 8x60K array slides.
|
| Description |
Gene expression of induced cancer stem cell induced from human iPS cell by ZR-75-1 cell culture medium
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 10.10.1.1 (Agilent Technologies) using parameters (protocol GE1_1010_Sep10 and Grid: 039494_D_F_20120628) to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
|
| |
|
| Submission date |
Apr 23, 2018 |
| Last update date |
Jul 01, 2018 |
| Contact name |
Akimasa Seno |
| Organization name |
Okayama University
|
| Department |
Applied Chemistry and Biotechnology
|
| Lab |
Nano-Biotechnology
|
| Street address |
1-1, Tsushima-Naka, 1-chome, Kita-Ku
|
| City |
Okayama |
| State/province |
Okayama |
| ZIP/Postal code |
7008530 |
| Country |
Japan |
| |
|
| Platform ID |
GPL17077 |
| Series (1) |
| GSE113533 |
Gene expression analysis of cancer stem cells with stem cells or cancer related cells |
|
