|
| Status |
Public on Mar 22, 2021 |
| Title |
2_CCCA |
| Sample type |
RNA |
| |
|
| Source name |
Scalp, affected
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: scalp
ccca status: Affected
patient id: 2
disease state: Central Centrifugal Cicatricial Alopecia
|
| Treatment protocol |
Samples submerged in 2mL tubes containing RNAlater stabilization reagent, stored at 4°C for 24hr, then at -80◦C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with the QIAGEN RNeasy Fibrous Tissue Mini Kit using manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
RNA was labeled with the Affymetrix GeneChip WT PLUS Reagent Kit using manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Hybridized for 17hrs at 45° C with rotation (60rpm) as described by Affymetrix in their GeneChip Expression Analysis Technical Manual.
|
| Scan protocol |
Using Affymetrix’ GeneChip Scanner 3000 7G and default parameters described by the manufacturer in their GeneChip Expression Analysis Technical Manual.
|
| Description |
Patient 2 Affected
|
| Data processing |
Affymetrix CEL files were extracted and their data normalized with the Partek GS 6.6 platform. RMA normalization created quantile-normalized log2 transcript-level signal values, which were used in teh subsequent ANOVA analysis. Transcript annotation was subsequently updated.
|
| |
|
| Submission date |
Apr 12, 2018 |
| Last update date |
Mar 23, 2021 |
| Contact name |
Crystal Aguh |
| E-mail(s) |
cagi1@jhmi.edu
|
| Organization name |
Johns Hopkins School of Medicine
|
| Department |
Dermatology
|
| Street address |
1500 Orleans St, CRB II Suite 200
|
| City |
Baltimore |
| State/province |
Maryland |
| ZIP/Postal code |
21224 |
| Country |
USA |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE113052 |
Gene Expression Profile of Central Centrifugal Cicatricial Alopecia |
|
