|
| Status |
Public on Nov 21, 2004 |
| Title |
Down syndrome heart CDH4 |
| Sample type |
RNA |
| |
|
| Source name |
Fetal heart at 21 weeks of gestation
|
| Organism |
Homo sapiens |
| Characteristics |
Heart tissue from fetus with Down syndrome and septal defects.
Age 21 gestation weeks
|
| Biomaterial provider |
Bank of fetal tissues at Federico II University - Naples
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extract by Trizol
|
| Label |
Biotin
|
| Label protocol |
In vitro transcription was performed to obtain biotin labeled cRNA according to Affymetrix protocols described at http://www.affymetrix.com/support/technical/manuals.affx
|
| |
|
| Hybridization protocol |
according to Affymetrix protocols described at http://www.affymetrix.com/support/technical/manuals.affx
|
| Scan protocol |
according to Affymetrix protocols described at http://www.affymetrix.com/support/technical/manuals.affx
|
| Description |
The heart tissue was explanted from a human fetus with chromosome 21 trisomy and atrio-ventricular septal defects at 21 weeks of gestation, following therapeutic abortion. The posterior half representing all the four heart chambers was dissected by a longitudinal cut. Total RNA was extracted and PolyA+ mRNA was obtained to prepare biotinilated target cRNA according to Affymetrix protocols (http://www.affymetrix.com/support/technical/manuals/expression_manual.affx). Target cRNA was hybridized to Affymetrix HG-U133A chip using an Affymetrix GeneChip Instrument System according to the procedures described at http://www.affymetrix.com/support/downloads/manuals/expression_s2_manual.pdf.
Image data were analyzed using the Affymetrix expression analysis software. The Affymetrix software Microarray Suite (MAS 5.0) assigned to each probe set of the chip an average difference value corresponding to the expression level of the gene it represents and an absolute call that indicates whether the transcript is present in the sample. Data sets were scaled to a total targeted fluorescence of 100.
Keywords = Down syndrome
Keywords = heart gene expression
|
| Data processing |
Affymetrix CEL files were imported into GeneSping 7.2 software (Agilent Technologies Inc.) and normalized by MAS5 algorithm
|
| |
|
| Submission date |
Sep 21, 2004 |
| Last update date |
Sep 30, 2024 |
| Contact name |
Lucio Nitsch |
| E-mail(s) |
nitsch@unina.it
|
| Phone |
+39-0817463621
|
| Fax |
+39-0817701016
|
| Organization name |
Unversity of Naples Federico II
|
| Department |
Biologia e Patologia Cellulare e Molecolare
|
| Street address |
Via Pansini 5
|
| City |
Napoli |
| ZIP/Postal code |
80131 |
| Country |
Italy |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE1789 |
heart gene expression in Down syndrome |
|
