|
| Status |
Public on Mar 13, 2018 |
| Title |
MDA-MB-231_LM2_1 |
| Sample type |
genomic |
| |
|
| Source name |
cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MDA-MB-231_LM2
cell type: breast cancer cell line
gfp: expresses GFP
|
| Treatment protocol |
Untreated cells
|
| Growth protocol |
Cells grown in DMEM 10% FBS, 1% P/S
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was made using Qiagen DNA mini kit and concentration determined using a Nanodrop Spectrophotometer
|
| Label |
Cy5/Cy3
|
| Label protocol |
Bisulfite conversion of genomic DNA samples was performed using the Zymo EZ DNA Methylation kit. 200ng of bisulfite-converted DNA was then prepared for hybridisation according to the Illumina Infinium HD Assay Methylation Protocol Guide.
|
| |
|
| Hybridization protocol |
Samples were hybridised to Illumina MethylationEPIC arrays for 17 hours at 48°C in an Illumina Hybridisation Oven. Single base extension and staining were performed as per the Illumina Infinium HD Protocol.
|
| Scan protocol |
Slides were scanned using an Illumina HiScan.
|
| Description |
SAMPLE 3
|
| Data processing |
Genomic DNA extracted from cell lines was genotyped using Infinium HumanCytoSNP-12 v2.1 BeadChip arrays (Illumina)
|
| |
|
| Submission date |
Mar 12, 2018 |
| Last update date |
Mar 13, 2018 |
| Contact name |
Andrew Pattison |
| E-mail(s) |
andrew.pattison@monash.edu
|
| Organization name |
Monash University
|
| Street address |
Wellington Rd
|
| City |
Melbourne |
| State/province |
Vic |
| ZIP/Postal code |
3800 |
| Country |
Australia |
| |
|
| Platform ID |
GPL13829 |
| Series (2) |
| GSE111705 |
Functional and genomic characterization of a xenograft model system for the study of metastasis in triple-negative breast cancer |
| GSE111706 |
Metastasis in triple-negative breast cancer |
|
