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Sample GSM3031436 Query DataSets for GSM3031436
Status Public on Oct 01, 2018
Title MI-FTC_tumor_rep2
Sample type RNA
 
Source name Tumorous region, MI-FTC, FFPE
Organism Homo sapiens
Characteristics tissue: Thyroid
gender: female
age: 51
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the NucleoSpin® totalRNA FFPE XS Kit (MACHEREY-NAGEL, Düren, Germany) according to the manufacturer's instructions. On-column DNaseI digestion was performed to avoid genomic DNA contamination.
Label biotin
Label protocol Biotinylated cRNA were prepared usibg the GeneChip® WT Pico Reagent Kit from 100 ng total RNA (GeneChip® WT Pico Reagent Kit User Manual, Affymetrix).
 
Hybridization protocol Fifteen ug of cRNA were subjected to fragmentation using the 3' fragmentation buffer hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the GeneChip® Scanner 3000 7G.
Description RNA from FFPE specimens
Data processing The data were analyzed with Affymetrix Transcriptome Analysis Console® using RMA as a summerization algorithm.
 
Submission date Mar 06, 2018
Last update date Oct 01, 2018
Contact name OSAMU ISHIBASHI
E-mail(s) ishibashi@biochem.osakafu-u.ac.jp
Phone +81-72-254-9474
Organization name Osaka Prefecture University
Department Grad Sch Life & Environmental Sciences
Lab Biological Macromolecules
Street address 1-1 Gakuen-cho, Naka-ku
City Sakai
ZIP/Postal code 599-8531
Country Japan
 
Platform ID GPL23126
Series (1)
GSE111455 Array-based genome-wide transcriptome analysis of Minimally invasive follicular thyroid carcinomas

Data table header descriptions
ID_REF
VALUE Bi-weight Avg Signal (log2)

Data table
ID_REF VALUE
TC0100006432.hg.1 2.8
TC0100006433.hg.1 3.71
TC0100006434.hg.1 4.05
TC0100006435.hg.1 3.13
TC0100006436.hg.1 3.75
TC0100006437.hg.1 3.62
TC0100006438.hg.1 6.55
TC0100006439.hg.1 4.81
TC0100006440.hg.1 3.03
TC0100006441.hg.1 4.08
TC0100006442.hg.1 3.34
TC0100006443.hg.1 2.98
TC0100006444.hg.1 3.43
TC0100006445.hg.1 5.17
TC0100006446.hg.1 4.17
TC0100006447.hg.1 17.07
TC0100006448.hg.1 3.23
TC0100006449.hg.1 3.69
TC0100006450.hg.1 3.7
TC0100006451.hg.1 4.49

Total number of rows: 135750

Table truncated, full table size 3044 Kbytes.




Supplementary file Size Download File type/resource
GSM3031436_701012-004_Clariom_D_Human_.CEL.gz 21.8 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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