|
| Status |
Public on Feb 06, 2018 |
| Title |
KMS-11 |
| Sample type |
SRA |
| |
|
| Source name |
KMS-11
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: KMS-11
cell type: Multiple myeloma cell line
|
| Growth protocol |
NCI-H929 cells were cultured in RPMI1640 medium containing 10%FBS and 0.05mM 2-Mercaptoethanol. U266B1 cells were cultured in RPMI1640 medium containing 15% FBS. RPMI8226, MM.1S, MM.1R, OPM2, KMM-1, KMS-11, KMS-20, KMS-21BM, KMS-26, KMS-27, KMS-28BM cells were cultured in RPMI1640 containing 10% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated and purified from cultured cells using an RNeasy Mini kit (QIAGEN).
A total of 10 ng RNA was reverse transcribed using the Ion AmpliSeq Transcriptome Human Gene Expression kit (Thermo Fisher Scientific) following the manufacturer’s protocol. cDNA libraries were amplified and barcoded using Ion AmpliSeq Transcriptome Human Gene Expression core panel and Ion Xpress Barcode Adapter (Thermo Fisher Scientific).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Ion Torrent Proton |
| |
|
| Data processing |
Base calling was performed using software provided with the Ion Torrent Proton sequencer (Torrent suite ver.5.0.4.0).
Sequenced reads were trimmed for adaptor sequence and masked for low-complexity or low-quality sequence, then mapped to hg19_AmpliSeq_Transcriptome_ERCC_v1 using software provided with the Ion Torrent Proton sequencer (AmpliSeqRNA v5.0.4.0).
RPM (reads per million) data were calculated using software provided with the Ion Torrent Proton sequencer (AmpliSeqRNA v5.0.4.0).
Genome_build: hg19
Supplementary_files_format_and_content: Tab-delimited text files include gene symbol and gene expression (read count) for each sample.
|
| |
|
| Submission date |
Feb 05, 2018 |
| Last update date |
Feb 06, 2018 |
| Contact name |
Kazuhide Nakayama |
| E-mail(s) |
kazuhide.nakayama@takeda.com
|
| Organization name |
Takeda Pharmaceutical Company Limited
|
| Department |
Regenerative medicine Unit
|
| Lab |
T-CiRA, Kaneko PJ
|
| Street address |
26-1
|
| City |
Muraoka-Higashi 2-chome Fujisawa |
| State/province |
Kanagawa |
| ZIP/Postal code |
251-8555 |
| Country |
Japan |
| |
|
| Platform ID |
GPL17303 |
| Series (1) |
| GSE110180 |
Transcriptomic analysis of multiple myeloma cell lines |
|
| Relations |
| BioSample |
SAMN08469689 |
| SRA |
SRX3651968 |
