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Sample GSM298063 Query DataSets for GSM298063
Status Public on Oct 01, 2008
Title Crown tissue of Harnesk at 9 weeks post germination, biological rep 1
Sample type RNA
Source name Harnesk crown 9 weeks post-germination (6 weeks vernalisation)
Organism Triticum aestivum
Characteristics variety: Harnesk
Grown for three weeks with 14 hour photoperiod; 16°C day 14°C night; PAR = 280 then an additional 6 weeks with declining temp, day-length and light intensity.
Growth protocol Seeds were planted in 50:50 potting compost:perlite in 10 cm pots and maintained in growth cabinets with a 14 h photoperiod (PAR 280) and 16°C day/14°C night. After three weeks of these conditions, plants were gradually exposed to a decline in temperature, day-length and light intensity: week 3-4 = 14 h photoperiod (PAR 280), 14°C day/12°C night; week 4-5 = 12 h photoperiod (PAR 185), 14°C day/10°C night;week 5--6 = 11 h photoperiod (PAR 185), 12°C day/10°C night;week 6-7 = 11 h photoperiod (PAR 185), 12°C day/8°C night;week 7-8 = 10 h photoperiod (PAR 95), 10°C day/6°C night;week 8-9 = 9 h photoperiod (PAR 95), 8°C day/4°C night;week 9-10 = 8 h photoperiod (PAR 95), 6°C day/2°C night;week 10-11 = 8 h photoperiod (PAR 95), 4°C day/2°C night;week 11-12 = 8 h photoperiod (PAR 48), 2°C day/2°C night.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Wheat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChip Wheat Genome Arrays wee scanned with the GeneChip 3000.
Description Plants 9 weeks post-germination; after 3 weeks' growth under standard conditions plants temperature, day-length and light intensity were reduced.
Data processing The data were analysed using GeneSpring GX 7.3: raw data was imported from .CEL files and normalised using RMA. Within GeneSpring GX, per gene normalisation to the median was perfored.
Submission date Jun 12, 2008
Last update date Jul 02, 2008
Contact name Mark Owen Winfield
Phone 07753191981
Organization name Bristol University
Department Biological Sciences
Lab OB120
Street address Woodland Road
City Bristol
ZIP/Postal code BS8 1UG
Country United Kingdom
Platform ID GPL3802
Series (1)
GSE11774 Expression data from cold treated wheat cultivars

Data table header descriptions
VALUE GeneSpring GX normalised value.

Data table
AFFX-BioB-3_at 0.934
AFFX-BioB-5_at 0.905
AFFX-BioB-M_at 0.866
AFFX-BioC-3_at 0.823
AFFX-BioC-5_at 0.848
AFFX-BioDn-3_at 0.904
AFFX-BioDn-5_at 0.860
AFFX-CreX-3_at 0.999
AFFX-CreX-5_at 1.006
AFFX-DapX-3_at 0.635
AFFX-DapX-5_at 0.707
AFFX-DapX-M_at 0.675
AFFX-LysX-3_at 0.502
AFFX-LysX-5_at 0.690
AFFX-LysX-M_at 0.786
AFFX-PheX-3_at 0.603
AFFX-PheX-5_at 0.626
AFFX-PheX-M_at 0.614
AFFX-r2-Bs-dap-3_at 0.634
AFFX-r2-Bs-dap-5_at 0.654

Total number of rows: 61290

Table truncated, full table size 1509 Kbytes.

Supplementary file Size Download File type/resource
GSM298063.CEL.gz 5.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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