|
| Status |
Public on Jul 18, 2019 |
| Title |
Placenta 6 |
| Sample type |
SRA |
| |
|
| Source name |
Placenta
|
| Organism |
Homo sapiens |
| Characteristics |
gestational age (weeks): 39
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
A fetal blood sample (0.2-2 ml) was collected from the umbilical cord into two EDTA-containing tubes after the delivery of the baby and before placental removal. The placental sampled area was taken from the midway between the point of cord insertion into the placenta and the placental circumference. DNA extraction was performed according to standard protocols(Qiagen kit).
RRBS libraries were prepared as described (Boyle, P. et al. 2012)
|
| |
|
| Library strategy |
Bisulfite-Seq |
| Library source |
genomic |
| Library selection |
Reduced Representation |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
100bpTiles_Tiles_Cov10_All.bed.zip
Cov10_Tiles_YoungWBC_More_Meth_0.50.xlsx
Cov10_Tiles_YoungPL_More_Meth_0.50.xlsx
Cov10_Tiles_OldWBC_More_Meth_0.50.xlsx
PL_6_N
|
| Data processing |
Adapter trimming using trim_galore
Read alignment and base calling: BSMAP v2.74
Tiles and DMRs calculation: MethylKit v0.9.1
Genome_build: hg19
Supplementary_files_format_and_content: Processed file is in bed format containing genome wide 100bp tiles methylation data of all samples
|
| |
|
| Submission date |
Jan 24, 2018 |
| Last update date |
Jul 18, 2019 |
| Contact name |
Tal Michaeli |
| Organization name |
The Hebrew University
|
| Street address |
Ein Kerem Campus
|
| City |
Jerusalem |
| ZIP/Postal code |
9112001 |
| Country |
Israel |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE109538 |
Determining gestational age using genome methylation profile |
|
| Relations |
| BioSample |
SAMN08389462 |
| SRA |
SRX3594420 |
