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Sample GSM2897545 Query DataSets for GSM2897545
Status Public on Jun 19, 2018
Title blood-HD-NS00026746-batch1-rep1
Sample type RNA
 
Source name HD_blood
Organism Homo sapiens
Characteristics diagnosis: HD
gender: famale
age: 54.04
cap score: 396.67
cap_d_group: high
tissue: blood
Extracted molecule total RNA
Extraction protocol Using PAXgene Blood RNA Kit(PreAnalytiX Cat#:762164) for RNA extraction following the manufaturer's protocol.
Label miRNA-specific probes
Label protocol Not applicable
 
Hybridization protocol 250 ng of total RNA was obtained from 233 samples, and hybridized to the nCounter Human miRNA sample probes (Seattle, WA).
Scan protocol Each sample was scanned for 600 FOV on the nCounter Digital Analyzer
Description Nanostring miRNA measurement for blood sample
NB00000211
Data processing We first loaded raw RCC files for 233 samples into nSolver (v2.5) and extracted the raw counts, then substracted background noise based on mean of negative controls, normalized to positive controls, finally performed global normalization using all miRNAs.
 
Submission date Dec 21, 2017
Last update date Jun 19, 2018
Contact name Xianjun Dong
E-mail(s) xianjun.dong.umass@gmail.com
Organization name Brigham and Women's Hospital
Street address 60 Kenwood Rd
City Boston
ZIP/Postal code 02115
Country USA
 
Platform ID GPL17904
Series (2)
GSE108395 Differential expression analysis of miRNAs expressed in blood and CSF of Huntington's disease [miRNA array]
GSE108398 Differential expression analysis of miRNAs expressed in blood and CSF of Huntington's disease

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
hsa-let-7a-5p 11147.40759
hsa-let-7b-5p 12036.43082
hsa-let-7c 631.2682307
hsa-let-7d-5p 2454.295032
hsa-let-7e-5p 37.26312644
hsa-let-7f-5p 398.4288135
hsa-let-7g-5p 2493.983569
hsa-let-7i-5p 2878.962378
hsa-miR-1 1.322951234
hsa-miR-100-5p 4.189345576
hsa-miR-101-3p 17.41885792
hsa-miR-103a-3p 6.835248045
hsa-miR-105-5p 1.322951234
hsa-miR-106a-5p+hsa-miR-17-5p 645.8206943
hsa-miR-106b-5p 1376.089776
hsa-miR-107 812.5125498
hsa-miR-10a-5p 35.9401752
hsa-miR-10b-5p 1.322951234
hsa-miR-1178 28.0024678
hsa-miR-1179 1.322951234

Total number of rows: 822

Table truncated, full table size 20 Kbytes.




Supplementary file Size Download File type/resource
GSM2897545_20150401_C10_C10_06.RCC.gz 7.1 Kb (ftp)(http) RCC
Raw data provided as supplementary file
Processed data included within Sample table

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