|
| Status |
Public on Dec 14, 2017 |
| Title |
Adult SPF Female Replicate 2 |
| Sample type |
SRA |
| |
|
| Source name |
Brain microglia
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
age: Adult (2 months)
antibiotics treatment: Specfic-pathogen-free (SPF)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA libraries were prepared for sequencing using SMART-Seq (TAKARA) protocole and Nextera XT DNA library (Illumina)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
SMARTseq + Nextera XT
A2M_SPF_Female
|
| Data processing |
reads were mapped to mouse reference genome GRCm38 using STAR. Reads per gene were counted using featureCounts. Mouse reference genome .fasta file and gene annotation .gtf file were downloaded from Gencode version M9
Genome_build: GRCm38
Supplementary_files_format_and_content: counts
|
| |
|
| Submission date |
Dec 13, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
jinmiao chen |
| E-mail(s) |
chen_jinmiao@immunol.a-star.edu.sg
|
| Organization name |
Singapore Immunology Network
|
| Street address |
8A Biomedical Grove, #04-06 Immunos
|
| City |
Singapore |
| ZIP/Postal code |
138648 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL19057 |
| Series (2) |
| GSE107130 |
Microbiome influences prenatal and adult microglia in a sex-specific |
| GSE108045 |
Genome wide study of adult microglia in Specific-pathogen-free (SPF) mice treated or not during one week with antibiotics (ABX) in males and females. |
|
| Relations |
| BioSample |
SAMN08173726 |
| SRA |
SRX3468858 |
