|
| Status |
Public on May 28, 2018 |
| Title |
Humanized mouse liver, control treatment, replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Liver from a chimeric mouse with a humanized liver, after 4 days control treatment
|
| Organism |
Mus musculus |
| Characteristics |
strain: PXB (chimeric mouse, with more than 70% of the liver replaced with normal human hepatocytes)
genotype/variation: cDNA-uPA+/wt/SCID, uPA+/wt; B6;129SvEv-Plau, SCID; C.B-17/Icr-scid/scid Jcl
Sex: male
treated with: vehicle only (0.5% hydroxypropyl methylcellulose)
tissue: Liver from a chimeric mouse with a humanized liver
|
| Treatment protocol |
Fenofibrate was dissolved in 0.5% hydroxypropyl methylcellulose (Shinestu Kagaku Kogyo, Japan) and administered orally to the mice at a dose of 300 mg/kg once per day for 4 days with a disposable plastic sonde (Fuchigami Kikai Co., Kyoto, Japan). Three mice received fenofibrate and three mice received the control treatment with vehicle only.
|
| Growth protocol |
The animal study was carried out in PXB mice (genotype: cDNA-uPA+/wt/SCID, uPA+/wt; B6;129SvEv-Plau, SCID; C.B-17/Icr-scid/scid Jcl) at PhoenixBio Co. Ltd. Briefly, cryopreserved human hepatocytes from a 2-year-old Hispanic girl were purchased from BD Biosciences (Woburn, MA, USA). After thawing, the hepatocytes were transplanted into hemizygous 2- to 4-week-old cDNA-uPA/SCID mice via the spleen under anesthesia. . A full description of the generation of these mice can be found elsewhere [PMID: 26536627]. Six male mice between 12-18 weeks of age were used for the study.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared from liver using TRIzol reagent, whereafter purified total RNA was isolated using Qiagen RNEasy columns. RNA integrity was checked on chip analysis (Agilent 2100 bioanalyzer, Agilent Technologies, Amsterdam, the Netherlands) according to the manufacturer's instructions. RNA was judged as suitable for array hybridization only if samples exhibited intact bands corresponding to the 18S and 28S ribosomal RNA subunits, and displayed no chromosomal peaks or RNA degradation products (RNA Integrity Number > 8.0)
|
| Label |
biotin
|
| Label protocol |
Purified total RNA (100ng per sample) was labeled with the Whole-Transcript Sense Target Assay (Affymetrix, Santa Clara, CA, USA; P/N 900652).
|
| |
|
| Hybridization protocol |
Hybridization and washing of the Affymetrix GeneChip Human Gene 1.1 ST peg arrays were performed on a GeneTitan Instrument (Affymetrix, Santa Clara, CA) according to the manufacturer's recommendations.
|
| Scan protocol |
Arrays were scanned on an Affymetrix GeneTitan instrument (Affymetrix, Santa Clara, CA).
|
| Description |
G283_G07_m_hLiver_102.CEL (veh)
|
| Data processing |
Expression estimates were calculated applying the RMA algorithm in the Bioconductor library 'Oligo' (v1.42.0).
|
| |
|
| Submission date |
Nov 16, 2017 |
| Last update date |
May 30, 2018 |
| Contact name |
Guido Hooiveld |
| E-mail(s) |
guido.hooiveld@wur.nl
|
| Organization name |
Wageningen University
|
| Department |
Div. Human Nutrition & Health
|
| Lab |
Nutrition, Metabolism & Genomics Group
|
| Street address |
HELIX, Stippeneng 4
|
| City |
Wageningen |
| ZIP/Postal code |
NL-6708WE |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL11532 |
| Series (1) |
| GSE107041 |
The whole genome effects of the PPARĪ± agonist fenofibrate on livers of hepatocyte humanized mice |
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