|
| Status |
Public on Jul 17, 2009 |
| Title |
WT dorsal skin at D17, biological replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse dorsal skin at 17 days after depilation, early anagen
|
| Organism |
Mus musculus |
| Characteristics |
Strain: C57BL/6
Age: at least postnatal day 50
Gender: Female
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 11 ug total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized on GeneChip Drosophila Genome Array using manufacturer's standard protocol. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using GeneChip 3000 Scanner 7G.
|
| Description |
Hair cycle phase: early anagen
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Apr 16, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Kevin K Lin |
| E-mail(s) |
kklin@alumni.uci.edu
|
| Organization name |
University of California, Irvine
|
| Department |
Biological Chemistry & Medicine
|
| Lab |
Bogi Andersen
|
| Street address |
250 Sprague Hall
|
| City |
Irvine |
| State/province |
CA |
| ZIP/Postal code |
92697-4030 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE11186 |
Expression profiling of mouse dorsal skin during hair follicle cycling |
|
| Relations |
| Reanalyzed by |
GSE119085 |
