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Sample GSM2753412 Query DataSets for GSM2753412
Status Public on Feb 27, 2018
Title 22RV1
Sample type SRA
Source name 22RV1_parental
Organism Homo sapiens
Characteristics tissue: prostate
cell line: 22RV1
treatment: control cells
Treatment protocol 22RV1 and LNcaP prostate cancer cells were cultured in RPMI1640 (Gibco) supplemented with 10% (v/v) FBS (Gibco). BETi (JQ1) resistant lines were developed by culturing in above mentioned media with IC50 concentration of the drug (100nM and 200nM for LNcaP and 22RV1 respectively) for over a period of 14-16 weeks.
Growth protocol Cells were grown in RPMI containing 10% FBS
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using miRNeasy Kit (Invitrogen) following the manufacturer’s instructions.
RNA libraries were prepared for sequencing using standard Illumina protocols
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
Data processing Illumina Nextseq500 reads were trimmed and clipped for quality control.
Read quality was checked for each sample using FastQC.
Alignment of reads were performed using STAR aligner with the default setting in Illumina Basespace (https://
Genome_build: hg19
Supplementary_files_format_and_content: FPKM values for each sample
Submission date Aug 24, 2017
Last update date May 15, 2019
Contact name Irfan A Asangani
Organization name University of Pennsylvania
Department Cancer Biology
Lab Asangani
Street address 421 Curie Blvd
City Philadelphia
State/province Pennylvania
ZIP/Postal code 19104
Country USA
Platform ID GPL18573
Series (1)
GSE103082 Resistance to BET inhibitor leads to new therapeutic vulnerabilities in castration resistant prostate cancer
SRA SRX3131036
BioSample SAMN07559489

Supplementary file Size Download File type/resource
GSM2753412_22RV1.genes.fpkm_tracking.gz 716.3 Kb (ftp)(http) FPKM_TRACKING
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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