|
| Status |
Public on Sep 04, 2008 |
| Title |
724 - mAdbID:70382 |
| Sample type |
RNA |
| |
|
| Source name |
724
|
| Organism |
Homo sapiens |
| Characteristics |
Gender: female
Age: 55
Tissue: lymph node
Disease state: Lymphoma
Individual: 724
Clinical info: Submitting diagnosis: DLBCL
Clinical info: Final microarray diagnosis: GCB DLBCL
Clinical info: Follow up status: DEAD
Clinical info: Follow up years: 1.4
Clinical info: Chemotherapy: CHOP-Like Regimen
Clinical info: ECOG performance status: 1
Clinical info: Stage: 2
Clinical info: LDH ratio: 1.16
Clinical info: Number of extranodal sites: 0
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Sample Extraction Protocol
Other: Total RNA was extracted using All Prep RNA/DNA kit (Qiagen, Valencia, CA) according to the manufacturers' protocols.
|
| Label |
biotin
|
| Label protocol |
Affymetrix Labeling Protocol
Other: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 microg mRNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Affymetrix Hybridization Protocol
Other: Following fragmentation, 11 micrograms of cRNA were hybridized for 16 hours at 45C on U133 plus 2.0 arrays from Affymetrix. Arrays were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Affy Scanning Protocol
Other: Scanning was performed by the Affymetrix 3000 Scanner.
|
| Description |
mAdb experiment ID: 70382
|
| Data processing |
MAS 5.0 Data Processing Protocol
Calculation Method: The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Mar 14, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Louis M. Staudt |
| E-mail(s) |
lstaudt@mail.nih.gov
|
| Phone |
301-402-1892
|
| Organization name |
National Cancer Institute
|
| Department |
Lymphoid Malignancies Branch
|
| Lab |
Louis M Staudt
|
| Street address |
9000 Rockville Pike, Bldg 10, Rm 4N114
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE10846 |
Prediction of survival in diffuse large B cell lymphoma treated with chemotherapy plus Rituximab |
| GSE11318 |
Molecular subtypes of DLBCL have distinct chromosomal aberrations |
|
| Relations |
| Reanalyzed by |
GSE119087 |
