|
| Status |
Public on Jul 31, 2019 |
| Title |
C2C12 cells, differentiation for 2 days, si-NC, replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse skeletal muscle cells
|
| Organism |
Mus musculus |
| Characteristics |
cell line: C2C12 cells
treatment: si-NC (control)
|
| Treatment protocol |
C2C12 cells were cultured in DMEM medium supplemented with 10% fetal bovine serum (FBS), and grown to 80-90% conflunce. Then, cells were transfected si-SYISL to knockdown SYISLand si-NC oligoes. And cells were transferred to DMEM medium contain 2% horse serum (HS), induced cells to differentiate for 2 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Trizol reagent (Invitrogen, USA) according to the manufacture, instructions
|
| Label |
Cy3
|
| Label protocol |
Sample labeling and array hybridization were performed according to the Agilent One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology). Briefly, 1 μg of total RNA from each sample was linearly amplified and labeled with Cy3-dCTP. The labeled cRNAs were purified by RNAeasy Mini Kit (Qiagen). The concentration and specific activity of the labeled cRNAs (pmol Cy3/μg cRNA) were measured by NanoDrop ND-1000.
|
| |
|
| Hybridization protocol |
1 μg of each labeled cRNA was fragmented by adding 11 μl 10 × Blocking Agent and 2.2 μl of 25 × Fragmentation Buffer, then heated the mixture at 60 °C for 30 min, finally 55 μl 2 × GE Hybridization buffer was added to dilute the labeled cRNA. 100 μl of hybridization solution was dispensed into the gasket slide and assembled to the lncRNA expression microarray slide. The slides were incubated for 17 hours at 65°C in an Agilent Hybridization Oven.
|
| Scan protocol |
After having washed the slides, the arrays were scanned by the Agilent Scanner G2505B.
|
| Description |
gene expression after transfected si-NC in C2C12 cells
|
| Data processing |
Agilent Feature Extraction software (version 10.5.1.1) was used to analyze acquired array images. Median normalization and subsequent data processing were performed using the GeneSpring GX v11.5.1 software package (Agilent Technologies).
|
| |
|
| Submission date |
Jul 31, 2017 |
| Last update date |
Jul 31, 2019 |
| Contact name |
lvwei jian jun |
| E-mail(s) |
jinjianjunai@126.com
|
| Phone |
13212798453
|
| Organization name |
huazhong agriculture university
|
| Department |
College of Animal science and technology
|
| Street address |
shizishan street, NO.1
|
| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430070 |
| Country |
China |
| |
|
| Platform ID |
GPL11202 |
| Series (1) |
| GSE102087 |
lncRNA SYISL contributes to the genome-wide expression during myogenic differentiation |
|
