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Sample GSM2695489 Query DataSets for GSM2695489
Status Public on Aug 09, 2017
Title MCF-7
Sample type SRA
 
Source name Breast cancer cell lines
Organism Homo sapiens
Characteristics subtype: Luminal
species: Human
Extracted molecule total RNA
Extraction protocol Total RNAs of each cell were isolated using the TRIzol reagent for RNA sequencing following manufacturer’s instructions. The total RNAs were treated with DNase I, purified with miRNeasy Mini Kit and subsequently quality checked using an Agilent 2100 Bioanalyzer.
RNA libraries were prepared for sequencing using standard Illumina protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Data processing An Illumina platform (Illumina) was used to analyze transcriptomes with 90 bp paired-end library.
Illumina Hiseq software used for basecalling.
Samples were paired-end sequenced with the Illumina HiSeq 2000.
Genome_build: mm9
Supplementary_files_format_and_content: text files include FPKM values for each Sample.
 
Submission date Jul 06, 2017
Last update date May 15, 2019
Contact name Seong-Jin Kim
E-mail(s) jasonsjkim@snu.ac.kr
Organization name Nano-Bio Medicine Research Center
Street address 145 Gwanggyo-ro, Yeongtong-gu,
City Suwon-si,
ZIP/Postal code 443-270
Country South Korea
 
Platform ID GPL11154
Series (1)
GSE100878 Gene expression profile in breast cancer cell lines using RNA sequencing
Relations
BioSample SAMN07327796
SRA SRX2987429

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data is available on Series record

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