|
| Status |
Public on Aug 09, 2017 |
| Title |
MCF-7 |
| Sample type |
SRA |
| |
|
| Source name |
Breast cancer cell lines
|
| Organism |
Homo sapiens |
| Characteristics |
subtype: Luminal
species: Human
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs of each cell were isolated using the TRIzol reagent for RNA sequencing following manufacturer’s instructions. The total RNAs were treated with DNase I, purified with miRNeasy Mini Kit and subsequently quality checked using an Agilent 2100 Bioanalyzer.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
An Illumina platform (Illumina) was used to analyze transcriptomes with 90 bp paired-end library.
Illumina Hiseq software used for basecalling.
Samples were paired-end sequenced with the Illumina HiSeq 2000.
Genome_build: mm9
Supplementary_files_format_and_content: text files include FPKM values for each Sample.
|
| |
|
| Submission date |
Jul 06, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Seong-Jin Kim |
| E-mail(s) |
jasonsjkim@snu.ac.kr
|
| Organization name |
Nano-Bio Medicine Research Center
|
| Street address |
145 Gwanggyo-ro, Yeongtong-gu,
|
| City |
Suwon-si, |
| ZIP/Postal code |
443-270 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE100878 |
Gene expression profile in breast cancer cell lines using RNA sequencing |
|
| Relations |
| BioSample |
SAMN07327796 |
| SRA |
SRX2987429 |
