|
| Status |
Public on Jul 30, 2019 |
| Title |
non-ADPKD 2 |
| Sample type |
RNA |
| |
|
| Source name |
non-ADPKD kidney tissue
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: non-ADPKD
tissue: kidney
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from kidney tissue samples of non-ADPKD and ADPKD patients using a Trizol method according to the manufacturer’s instructions (Invitrogen Life Technologies).
|
| Label |
none
|
| Label protocol |
Per RNAsample, 100 ng were used as input into the Agilent procedure as recommended by protocol (miRNA Microarray System with miRNA Complete Labeling and Hyb Kit protocol 2.1).
|
| |
|
| Hybridization protocol |
100 ng of total RNA was used as an input into the labeling reaction, and labeled miRNA was hybridized to the array for 20 h at 55°C and 20 rpm, as described in the manufacturer’s protocol. After hybridization, the chips were washed and scanned using the Agilent DNA Microarray Scanner.
|
| Scan protocol |
Global miRNA gene expression analyses were performed on the Agilent Human miRNA Microarray Version 3 software (Agilent design IDs 021827) according to the manufacturer’s instructions (miRNA Microarray System with miRNA Complete Labeling and Hyb Kit protocol 2.1).
|
| Description |
non-ADPKD renal tissue samples were obtained from patients undergoing surgery for clear cell renal cell carcinoma, where malignant cell infiltration was excluded by histology.
PKD_Normal_rep2
|
| Data processing |
The scanned images were analyzed with GeneSpring GX11 TMEV4.1.
|
| |
|
| Submission date |
Jul 05, 2017 |
| Last update date |
Jul 30, 2019 |
| Contact name |
Sumin Oh |
| E-mail(s) |
kstori3924@gmail.com
|
| Phone |
82-10-7203-2307
|
| Organization name |
Sookmyung Women's University
|
| Department |
Department of Biological Science
|
| Lab |
LBG
|
| Street address |
Cheongpa-ro 47-gil 100, Yongsan-gu
|
| City |
Seoul |
| ZIP/Postal code |
04310 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL18743 |
| Series (1) |
| GSE100812 |
Expression patterns of key miRNAs in end-stage ADPKD kidneys |
|
