|
| Status |
Public on Jun 19, 2017 |
| Title |
F_P14 8 |
| Sample type |
SRA |
| |
|
| Source name |
female_P14_untreated_microglia
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6N
Sex: female
developmental stage: P14
treated with: none (untreated)
tissue: microglia
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
RNA libraries were prepared for sequencing using standard Illumina protocols for the stranded polyA high-throughput kit.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
[[B5]]_tube34_P14_3.14.14_F-4
FGC0900_s_2_ATTCAGAAATAGAGGC
|
| Data processing |
Illumina Casava-1.8.2 software was used for basecalling.
RNA-Seq - Low quality reads as well as ribosomal and other repeat sequences were filtered out before alignment. RUM software was used for aligning reads to mouse genome mm9 and Refseq transcriptome and for transcript quantitation. Differential expression analysis was carried out using a custom script implementing EdgeR software.
Genome_build: mm9
Supplementary_files_format_and_content: Excel format file of transcript raw read counts of each sample
|
| |
|
| Submission date |
Jun 02, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
mark alter |
| E-mail(s) |
markalter1968@gmail.com
|
| Organization name |
University of Pennsylvania
|
| Department |
Center for Neurobiology and Behvior
|
| Street address |
125 S. 31st Street
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19003 |
| Country |
USA |
| |
|
| Platform ID |
GPL17021 |
| Series (1) |
| GSE99622 |
Sex differences in microglial developmental gene expression programs |
|
| Relations |
| BioSample |
SAMN07189897 |
| SRA |
SRX2880637 |
