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Sample GSM2643541 Query DataSets for GSM2643541
Status Public on Dec 06, 2017
Sample type SRA
Source name GM15850 LCLs (FRDA)
Organism Homo sapiens
Characteristics antibody: BRD4
cell line: GM15850 LCLs (FRDA)
treatment: 0.1% DMSO
treatment time: 24 h
Treatment protocol GM15850 cells were treated with the indicated molecules for 24 h
Growth protocol RPMI 1640 Medium with 2 mM L-glutamine and 15% FBS
Extracted molecule genomic DNA
Extraction protocol Cells were chemically crosslinked by the addition of 4 mL of 1.5% formaldehyde in PBS per 10 cm2 dish for 15 min at room temperature. Cells were resusupended in Lysis Buffer 1 (50 mM HEPES , pH 7.5, 140 mM NaCl, 1 mM EDTA, 10% glycerol, 0.5% IGEPAL, 0.25% Triton X-100, 1X HALT protease inhibitor) and 3T3 mouse embryonic fibroblast cells (formaldehyde fixed as above) equivalent to 10% GM15850 cells were added to each sample for downstream ChIP-RX analysis.
NGS libraries were generated with Thruplex DNAseq Single Index Kit (Rubicon) and sequenced (single end, 150 bp reads) via NextSeq 500.
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina NextSeq 500
Data processing n/a
Genome_build: Human: GRCh37 (hg19) Mouse: mm9
Submission date May 30, 2017
Last update date May 15, 2019
Contact name Aseem Ansari
Organization name University of Wisconsin-Madison
Department Biochemistry and The Genome Center
Lab Ansari
Street address 440 Henry Mall
City Madison
State/province WI
ZIP/Postal code 53706
Country USA
Platform ID GPL18573
Series (2)
GSE99402 ChIP-RX of GM15850 cells treated with synthetic transcription elongation factors
GSE99403 Synthetic transcription elongation factors
BioSample SAMN07176164
SRA SRX2867630

Supplementary data files not provided
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Processed data not provided for this record
Raw data are available in SRA

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