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Sample GSM2635755 Query DataSets for GSM2635755
Status Public on Nov 15, 2017
Title Input
Sample type SRA
Source name HaCaT cell line
Organism Homo sapiens
Characteristics cell line: HaCaT
Treatment protocol NA
Growth protocol HaCaT cells were cultured in Dulbecco’s Modified Eagle Medium supplemented with 10% FBS.
Extracted molecule genomic DNA
Extraction protocol Chromatin was isolated from HaCaT cells that were cross-linked using 1% formaldehyde for 10 min at RT and prepared using a ChIP-qPCR Kit (Chromatrap, cat. no. 500117). Chromatin was sonicated using a Bioruptor Plus (Diagenode) to give a fragment distribution of 100-500 bp. For further details see methods section in the associated manuscript.
G4 ChIPed DNA was subjected to Nextera library preparation (Illumina, cat. no. FC-121-1030). For further details see methods section in the associated manuscript.
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina NextSeq 500
Data processing The data was processed as described in the following paper Details about the analysis can also be found at
Genome_build: hg19
Supplementary_files_format_and_content: For narrowPeak format see MACS2 documentation at
Submission date May 23, 2017
Last update date May 15, 2019
Contact name Giovanni Marsico
Organization name CRUK Cambridge Institute
Street address Robinson Way
City Cambridge
ZIP/Postal code CB2 0RE
Country United Kingdom
Platform ID GPL18573
Series (1)
GSE99205 Chromatin immunoprecipitation for genome-wide mapping of endogenous G-quadruplex DNA structures
BioSample SAMN07157021
SRA SRX2844872

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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