|
| Status |
Public on Apr 06, 2008 |
| Title |
ATH-AGO2 |
| Sample type |
SRA |
| |
|
| Source name |
Immunopurified Arabidopsis AGO2 complex
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
Small RNAs that are associated with Arabidopsis AGO2 complex
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Small RNAs were extracted from immunopurified AGO complexes using Trizol reagent
|
| |
|
| Library strategy |
OTHER |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
Illumina Genome Analyzer |
| |
|
| Description |
Small RNAs were prepared from Arabidopsis AGO1, AGO2, AGO4 and AGO4 complexes, ligated to a 3' adaptor and a 5' acceptor sequentially, and then RT-PCR amplified. PCR products were reamplified using a pair of solexa cloning primers and then provided for sequencing.
|
| Data processing |
The adaptor sequences were removed using “vectorstrip” in EMBOSS package. The sRNA reads with length of 19-27 nt were mapped to the Arabidopsis nuclear, chloroplast and mitochondrial genomes (http://www.arabidopsis.org/). The sRNAs with perfect genomic matches are used for further analysis
|
| |
|
| Submission date |
Dec 27, 2007 |
| Last update date |
Jun 11, 2013 |
| Contact name |
Tao Cai |
| E-mail(s) |
caitao@nibs.ac.cn
|
| Organization name |
National Institute Of Biological Sciences, Beijing (NIBS)
|
| Lab |
Sequencing Facility
|
| Street address |
No. 7 Science Park Road, Zhongguancun Life Science Park
|
| City |
Beijing |
| ZIP/Postal code |
102206 |
| Country |
China |
| |
|
| Platform ID |
GPL9062 |
| Series (1) |
| GSE10036 |
Small RNAs in four Argonaute complexes in Arabidopsis |
|
| Relations |
| BioSample |
SAMN02195384 |
