|
| Status |
Public on Mar 09, 2017 |
| Title |
ChIP-seq from neutrophil (ENCLB642JUJ) |
| Sample type |
SRA |
| |
|
| Source name |
Homo sapiens neutrophil primary cell
|
| Organism |
Homo sapiens |
| Characteristics |
antibody: H3K27ac
cell_type: neutrophil
biomaterial_type: primary cell
description: Neutrophils were isolated by centrifuging heparinized venous blood over Histopaque 1119 (Sigma-Aldrich) and subsequently over a discontinuous Percoll (Amersham Biosciences) gradient. Cells provided by Max Planck Institute for Infection Biology, Berlin
biosample encode accession: ENCBS921VGK (SAMN06464560)
dev stage: unknown
Sex: not collected
donor_id: ENCDO055UKA
age: unknown
lab: Bradley Bernstein, Broad
link: ENCBS921VGK at ENCODE; https://www.encodeproject.org/ENCBS921VGK/
link: Derived from ENCODE donor ENCDO055UKA; https://www.encodeproject.org/ENCDO055UKA/
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
general protocol: https://www.encodeproject.org/documents/f35827f6-da4a-42a6-b4bd-9e1b8b6c1663/@@download/attachment/Epigenomics_Alternative_Mag_Bead_ChIP_Protocol_v2.0.pdf
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
https://www.encodeproject.org/experiments/ENCSR267YXV/
***************
biological replicate number: 1
technical replicate number: 1
description:
experiment encode accession: ENCSR267YXV
assay title: ChIP-seq
assembly: GRCh38, hg19
possible controls: ENCSR557RDB
encode release date: 2016-11-16
lab: Bradley Bernstein, Broad
library encode accession: ENCLB642JUJ
size range: 200-600
|
| Data processing |
See GSM*_README.txt supplementary file linked below
|
| |
|
| Submission date |
Mar 09, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
ENCODE DCC |
| E-mail(s) |
encode-help@lists.stanford.edu
|
| Organization name |
ENCODE DCC
|
| Street address |
300 Pasteur Dr
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5120 |
| Country |
USA |
| |
|
| Platform ID |
GPL16791 |
| Series (1) |
| GSE96014 |
ChIP-seq from neutrophil (ENCSR267YXV) |
|
| Relations |
| BioSample |
SAMN06464560 |
| SRA |
SRX2630391 |
