|
| Status |
Public on Sep 01, 2017 |
| Title |
HepG2_BFA_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
HepG2, BFA
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: liver
cell line: HepG2
agent: BFA
|
| Treatment protocol |
HepG2 cells were treated with DMSO or Brefeldin A (300 nM) for 24 hours.
|
| Growth protocol |
HepG2 cells were grown in EMEM supplemented with 10% FBS (Thermo Fisher Scientific) and antibiotic/antimycotic solution (Sigma).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Plus Mini kit (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
200 μg of total RNA were amplified and labeled using the Low Input QuickAmp Labeling kit (Agilent Technologies) according to the manufacturer’s protocols.
|
| |
|
| Hybridization protocol |
600 ng of Cy3-labeled RNA was hybridized at 65⁰C for 17 hours.
|
| Scan protocol |
The signals were detected by using the DNA microarray scanner (Agilent Technologies).
|
| Description |
Gene expression in HepG2 cells treated with BFA
|
| Data processing |
The scanned images were extracted with Feature Extraction software (Version 10.7.3.1; Agilent Technologies) using default parameters.
|
| |
|
| Submission date |
Feb 14, 2017 |
| Last update date |
Sep 01, 2017 |
| Contact name |
Kiyoshi Yamaguchi |
| Organization name |
The University of Tokyo
|
| Department |
Division of Clinical Genome Research
|
| Street address |
4-6-1 Shirokanedai, Minato-ku
|
| City |
Tokyo |
| ZIP/Postal code |
108-8639 |
| Country |
Japan |
| |
|
| Platform ID |
GPL17077 |
| Series (1) |
| GSE94861 |
Gene expression profiling of HepG2 cells after treatment with Brefeldin A |
|
