|
| Status |
Public on Apr 21, 2017 |
| Title |
CD1C_P3_S14 |
| Sample type |
SRA |
| |
|
| Source name |
PBMC
|
| Organism |
Homo sapiens |
| Characteristics |
sorted_gate_identity: CD1C
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Enriched immune cell fractions isolated from healthy blood PBMCs were FACS sorted in 96-well plates (single cell sorted) containing lysis buffer (TCL together with 1% of 2-Mercaptoethanol).
Smart-seq2 (Picelli et al., Nature Methods, 2014)
Full-length RNA-sequencing
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
paired-end RNA-seq data
processed data file:
raw.expMatrix_DCnMono.discovery.set.submission.txt
|
| Data processing |
Read were aligned to the UCSC hg19 transcriptomee using Bowtie v0.12.7
Expression levels were quantified using RSEM v1.2.1 (TPM values)
UCSC genome table browser was used to map UCSC gene ID (kgID) and the gene name (geneSymbol) for all genes in hg19
If multiple UCSC gene IDs are assigned to the same geneSymbol, the TPM values of all kgIDs that share the same geneSymbol are summed
Genome_build: hg19
Supplementary_files_format_and_content: Text file with tab delimiters
|
| |
|
| Submission date |
Feb 13, 2017 |
| Last update date |
Apr 21, 2017 |
| Contact name |
Nir Hacohen |
| Organization name |
Broad Institute
|
| Lab |
Nir Hacohen
|
| Street address |
415 Main Street
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platform ID |
GPL16791 |
| Series (1) |
| GSE94820 |
Single-cell RNA-seq reveals new types of human blood dendritic cells, monocytes and progenitors |
|
| Relations |
| BioSample |
SAMN06324755 |
