|
| Status |
Public on Oct 03, 2018 |
| Title |
Alstonville Protein Rep1 |
| Sample type |
SRA |
| |
|
| Source name |
Third instar larvae
|
| Organism |
Drosophila melanogaster |
| Characteristics |
diet protein: carb ratio: 1:2
mitotype: Alstonville
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Sorted cells were preserved in RNAlater (ThermoFisher Scientific) and frozen at -80°C, then RNA was isolated with RNeasyPlus Mini kit (Qiagen)
mRNA reverse transcription and cDNA libraries were prepared using the TruSeq RNA Sample preparation kit (Illumina) following the manufacturer’s instructions
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Indexed sample libraries were sequenced using the 75 cycle high output kit v2 chemistry for the Illumina Hiseq2000 sequencing instrument
Reads were aligned to the mouse genome by Rsubread version 1.16.1
Read counts were summarized by Entrez Gene ID using Rsubread featureCounts function and NCBI annotation
Genome_build: GCF 000001215.4 Release 6 plus ISO1 MT
Supplementary_files_format_and_content: tab-delimited text files were written from the R prompt; columns represent Entrez Gene ID, total gene length (total base count of all exons) and the number of reads mapping to that gene
|
| |
|
| Submission date |
Jan 18, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Gordon K Smyth |
| E-mail(s) |
smyth@wehi.edu.au
|
| Phone |
(+61 3) 9345 2326
|
| Fax |
(+61 3) 9347 0852
|
| URL |
http://www.wehi.edu.au
|
| Organization name |
Walter and Eliza Hall Institute of Medical Research
|
| Department |
Bioinformatics
|
| Lab |
Smyth
|
| Street address |
1G Royal Pde
|
| City |
Parkville |
| State/province |
Vic |
| ZIP/Postal code |
3052 |
| Country |
Australia |
| |
|
| Platform ID |
GPL13304 |
| Series (1) |
| GSE93806 |
Metabolic flexibility enables positive selection of mtDNA in Drosophila (RNA-seq) |
|
| Relations |
| BioSample |
SAMN06240241 |
| SRA |
SRX2502083 |
