|
| Status |
Public on Aug 08, 2017 |
| Title |
0h LPS rep1 |
| Sample type |
SRA |
| |
|
| Source name |
0h LPS
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
cell type: Bone marrow derived macrophages
treatment: 0h LPS
|
| Treatment protocol |
Primary macrophages derived from bone marrow were stimulated with lipopolysaccharide and/or Dex for the indicated number of hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from approximately 1x10e6 cells by using an RNAeasy Mini Kit (Qiagen).
Sequencing libraries were generated using Illumina TruSeq V3 protocol and subject to 101 bp paired-end sequencing on Illumina HiSeq2000.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
GH1280
|
| Data processing |
Base-calling with Illumina run time analysis software 1.12.4.2
Alignment to mm9 using Casava Eland extended
Transcript levels were quantified using Tophat and Cufflinks.
Genome_build: mm9
Supplementary_files_format_and_content: FPKM
|
| |
|
| Submission date |
Jan 17, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Songjoon Baek |
| Organization name |
NCI / NIH
|
| Department |
CCR
|
| Lab |
LRBGE
|
| Street address |
41 Library Drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL13112 |
| Series (2) |
| GSE93735 |
Anti-inflammatory Chromatinscape Associated with Clinically Relevant Timing of Glucocorticoid Treatment [RNA-Seq] |
| GSE93739 |
Anti-inflammatory Chromatinscape Associated with Clinically Relevant Timing of Glucocorticoid Treatment |
|
| Relations |
| BioSample |
SAMN06236679 |
| SRA |
SRX2498764 |
