|
| Status |
Public on Jan 20, 2017 |
| Title |
Het-4 |
| Sample type |
SRA |
| |
|
| Source name |
Cerebellar granule neuron progenitors
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: cerebellum
developmental stage: P7
cell type: Granule Neuron Progenitors
genotype: Atoh1-Cre::Chd7 f/+
|
| Treatment protocol |
Cells were freshly isolated from P7 pups
|
| Growth protocol |
Primary cells without culture
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tri-Reagent combined with QIAGEN RNAeasy Kit
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
RSEM-1.2.15 with Bowtie-1.0.0 is used for mapping, default setting is used.
ComBat with default setting was used to remove batch effect
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include TPM values for each Sample ...
|
| |
|
| Submission date |
Jan 17, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Haikun Liu |
| E-mail(s) |
l.haikun@dkfz.de
|
| Organization name |
DKFZ
|
| Street address |
INF 581
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL13112 |
| Series (2) |
| GSE72207 |
RNA-seq of Chd7 wild type and mutant granule neuron progenitors |
| GSE93741 |
Chd7 is indispensable for mammalian brain development through activation of a neuronal differentiation program |
|
| Relations |
| BioSample |
SAMN06236697 |
| SRA |
SRX2498781 |
