tissue: whole blood disease state: control gender: male survival (months): 34 age (years): 72 fvc: NA dlco: NA composite_end_point: 0
Extracted molecule
total RNA
Extraction protocol
The PAXgene Blood RNA Kit (PreAnalytiX) was used to isolate RNA according to the manufacturer’s protocol.
Label
biotin
Label protocol
Samples were fragmented and labelled using the Encore® Biotin Module Kit (NuGEN)
Hybridization protocol
Samples were hybridized using Affymetrix hybridization kit material.
Scan protocol
Affymetrix GeneTitan
Description
Control, Timepoint 0
Data processing
Data were processed using Affymetrix Power Tools. RMA background correction were applied to probe intensities, gene level expression were summarized from the intensities and then quantile normalized.
