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GEO accession:
Sample GSM2453365
Query DataSets for GSM2453365
Status
Public on Feb 03, 2017
Title
polyA mRNA RNA-seq from ES-Bruce4 (ENCLB331QIU)
Sample type
SRA
Source name
Mus musculus strain Bruce4 ES-Bruce4 stem cell male embryo
Organism
Mus musculus
Characteristics
cell_type: ES-Bruce4
biomaterial_type: stem cell
description: mouse embryonic stem cells
biosample encode accession: ENCBS250IKX (SAMN06216019)
dev stage: embryonic
donor_id: ENCDO081AAA
age: unknown
Sex: male
lab: Bing Ren, UCSD
link: ENCBS250IKX at ENCODE;
https://www.encodeproject.org/ENCBS250IKX/
link: Derived from ENCODE donor ENCDO081AAA;
https://www.encodeproject.org/ENCDO081AAA/
link: ENCODE dbxrefs Cellosaurus CVCL_K037;
http://web.expasy.org/cellosaurus/CVCL_K037
link: ENCODE dbxrefs ES-Bruce4;
http://genome.cse.ucsc.edu/cgi-bin/hgEncodeVocab?ra=encode/cv.ra&term=ES-Bruce4
Extracted molecule
total RNA
Extraction protocol
general protocol:
https://www.encodeproject.org/documents/d44ce49b-2379-4359-99e0-2272a5dcd136/@@download/attachment/wgEncodeLicrRnaSeq.release2.html.pdf
Library strategy
RNA-Seq
Library source
transcriptomic
Library selection
cDNA
Instrument model
Illumina HiSeq 2000
Description
https://www.encodeproject.org/experiments/ENCSR000CGU/
***************
biological replicate number: 2
technical replicate number: 1
description: RNA-seq on embryonic mouse stem cell line Bruce4
experiment encode accession: ENCSR000CGU
assay title: polyA mRNA RNA-seq
assembly: mm10
encode release date: 2011-08-04
lab: Bing Ren, UCSD
library encode accession: ENCLB331QIU
size range: >200
spikeins used: /references/ENCSR866KGS/
Data processing
See GSM*_README.txt supplementary file linked below
Submission date
Jan 11, 2017
Last update date
May 15, 2019
Contact name
ENCODE DCC
E-mail(s)
encode-help@lists.stanford.edu
Organization name
ENCODE DCC
Street address
300 Pasteur Dr
City
Stanford
State/province
CA
ZIP/Postal code
94305-5120
Country
USA
Platform ID
GPL13112
Series (1)
GSE93453
polyA mRNA RNA-seq from ES-Bruce4 (ENCSR000CGU)
Relations
BioSample
SAMN06216019
SRA
SRX2487425
Supplementary file
Size
Download
File type/resource
GSM2453365_ENCFF045VKH_signal_of_unique_reads_mm10.bigWig
79.1 Mb
(ftp)
(http)
BIGWIG
GSM2453365_ENCFF069VAH_signal_of_all_reads_mm10.bigWig
135.6 Mb
(ftp)
(http)
BIGWIG
GSM2453365_ENCFF104TTL_gene_quantifications_mm10.tsv.gz
2.6 Mb
(ftp)
(http)
TSV
GSM2453365_ENCFF353UDS_transcript_quantifications_mm10.tsv.gz
5.1 Mb
(ftp)
(http)
TSV
GSM2453365_ENCLB331QIU_README.txt.gz
601 b
(ftp)
(http)
TXT
SRA Run Selector
Raw data are available in SRA
Processed data provided as supplementary file
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