|
| Status |
Public on Apr 11, 2017 |
| Title |
6_dehydration2 |
| Sample type |
SRA |
| |
|
| Source name |
leaf tissue
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
tissue: leaf
genotype: wrky6
treatment: Dehydration
replication: 2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was then extracted using Trizol, followed by DNAse digestion and RNA cleanup using Qiagen RNeasy kit. Purified RNA was subject to quality control on an Agilent 2100 Bioanalyzer.
Library preparation and RNA sequencing were performed by BGI Americas using an Illumina HiSeq 2000 with 50bp single-end reads and ~30 million reads per sample.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
Sample name: 52
|
| Data processing |
Quality Control
Alignment using GSNAP
Differential expression using DESeq2
Genome_build: TAIR10
Supplementary_files_format_and_content: comma seperated files including RPM values and statistical significance for each sample calculated using DESeq2
|
| |
|
| Submission date |
Jan 11, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Yanhai Yin |
| E-mail(s) |
yin@iastate.edu
|
| Organization name |
Iowa State University
|
| Department |
Genetic Development and Cell Biology
|
| Street address |
1035D Roy J. Carver Co-Lab
|
| City |
Ames |
| State/province |
IA |
| ZIP/Postal code |
50011-3650 |
| Country |
USA |
| |
|
| Platform ID |
GPL13222 |
| Series (1) |
| GSE93420 |
Dehydration and Fixed-Carbon Starvation of Brassinosteroid related mutants in Arabidopsis |
|
| Relations |
| BioSample |
SAMN06216760 |
| SRA |
SRX2484768 |
