|
| Status |
Public on Jan 10, 2017 |
| Title |
BEAS-2B cells as control, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
BEAS-2B cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: bronchial epithelial cells
treatment: DMEM
|
| Treatment protocol |
Suspension of PM2.5 was dispersed by sonicator, and diluted to 50 μg/ml, then add to BEAS-2B cells immediately.Cells maintained in DMEM culture medium without PM2.5 were used as control group.
|
| Growth protocol |
BEAS-2B cells were seeded in petri dishes at density of 1×105 cells/mL and allowed to attach for 24 h, then treated with PM2.5 suspended in DMEM culture medium or DMEM culture mediun only for another 24 h.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
cells as normal control
|
| Data processing |
The data were analyzed with RMA using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Jan 09, 2017 |
| Last update date |
Jan 10, 2017 |
| Contact name |
唐 伟评 |
| Organization name |
Beijing Institute of Heart Lung and Blood Vessel Diseases
|
| Street address |
2 Anzhen Rd, Chaoyang District,
|
| City |
Beijing |
| ZIP/Postal code |
100029 |
| Country |
China |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE93329 |
Expression data from BEAS-2B cells |
|
