|Public on Nov 22, 2008
|Mixed infected tissue, biological rep3
|poplar leaf tissues infected both with Mlp and Mmd
|Populus maximowiczii x Populus nigra
|Fully expanded leaves from leaf plastochron index (LPI) five and six were detached and infected with Mlp and Mmd at a density of 2,000 spores per cm2 each.
|Poplar leaves were detached from trees and seperated to half-leaves. Half-leaves were kept on wet paper on their abaxial side inside Petri dishes prior to infection.
|Poplar clone NM6 was grown grown in Promix soil under controlled greenhouse conditions prior to inoculation. NM6 plants were kept under a 16-h photoperiod with weekly fertilizations (20:20:20 N: P: K). Melampsora larici-populina (Mlp) and Melampsora medusae f. sp. deltoidae (Mmd) were maintained on clone Jackii (Populus balsamifera L. x Populus deltoides Marsh.).
|Total RNA were extracted from frozen leaf tissues using the RNAeasy Plant Mini Kit (Qiagen, Mississauga, ON) according to the manufacturer’s instructions. Following a DNase I treatment (Qiagen), 10 μg of total RNA from each biological sample were used for each microarray experiment.
|Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Poplar Genome Array. GeneChips were washed and stained in the Fluidics protocol EukGE_WS2v5_450
|Gene expression data from infected tissues with both Melampsora larici poplulina and Melampsora medusae f.sp. deltoides
|The data were analyzed with Robust Multichip Average (RMA)
|Nov 23, 2007
|Last update date
|Aug 14, 2011
|Laurentian forestry Service
|1055 rue de PEPS
|Comparative transcriptomic reveals signatures specific to various interactions with Melampsora rust fungi