|
| Status |
Public on Nov 22, 2008 |
| Title |
Mixed infected tissue, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
poplar leaf tissues infected both with Mlp and Mmd
|
| Organism |
Populus maximowiczii x Populus nigra |
| Characteristics |
Fully expanded leaves from leaf plastochron index (LPI) five and six were detached and infected with Mlp and Mmd at a density of 2,000 spores per cm2 each.
|
| Treatment protocol |
Poplar leaves were detached from trees and seperated to half-leaves. Half-leaves were kept on wet paper on their abaxial side inside Petri dishes prior to infection.
|
| Growth protocol |
Poplar clone NM6 was grown grown in Promix soil under controlled greenhouse conditions prior to inoculation. NM6 plants were kept under a 16-h photoperiod with weekly fertilizations (20:20:20 N: P: K). Melampsora larici-populina (Mlp) and Melampsora medusae f. sp. deltoidae (Mmd) were maintained on clone Jackii (Populus balsamifera L. x Populus deltoides Marsh.).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA were extracted from frozen leaf tissues using the RNAeasy Plant Mini Kit (Qiagen, Mississauga, ON) according to the manufacturer’s instructions. Following a DNase I treatment (Qiagen), 10 μg of total RNA from each biological sample were used for each microarray experiment.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Poplar Genome Array. GeneChips were washed and stained in the Fluidics protocol EukGE_WS2v5_450
|
| Scan protocol |
GeneChip scanner
|
| Description |
Gene expression data from infected tissues with both Melampsora larici poplulina and Melampsora medusae f.sp. deltoides
|
| Data processing |
The data were analyzed with Robust Multichip Average (RMA)
|
| |
|
| Submission date |
Nov 23, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Armand Séguin |
| E-mail(s) |
Armand.Seguin@rncan.gc.ca
|
| Phone |
418-648-2533
|
| Organization name |
Laurentian forestry Service
|
| Street address |
1055 rue de PEPS
|
| City |
Québec |
| State/province |
Québec |
| ZIP/Postal code |
G1V4C7 |
| Country |
Canada |
| |
|
| Platform ID |
GPL4359 |
| Series (1) |
| GSE9673 |
Comparative transcriptomic reveals signatures specific to various interactions with Melampsora rust fungi |
|
