|
| Status |
Public on Jan 01, 2018 |
| Title |
HEC1A-sh KD |
| Sample type |
RNA |
| |
|
| Source name |
knockdown of EMP2
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HEC1A
genotype/variation: EMP2 knockdown
|
| Treatment protocol |
untreated
|
| Growth protocol |
McCoys + 10% FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen total RNA purification
|
| Label |
biotin
|
| Label protocol |
Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Samples were hybridized using Affymetrix hybridization kit materials
|
| Scan protocol |
Standard Affymetrix protocol.
GeneChip Scanner 3000 7G
Scanner type: M10
Hardware version: 1.0
Software version: 4.0.0.16
|
| Data processing |
Data were analyzed with Partek genomics Suite 6.6
|
| |
|
| Submission date |
Dec 23, 2016 |
| Last update date |
Jan 01, 2018 |
| Contact name |
Madhuri Wadehra |
| E-mail(s) |
mwadehra@mednet.ucla.edu
|
| Phone |
310-825-1590
|
| Organization name |
UCLA
|
| Department |
Pathology and Lab Medicine
|
| Street address |
675 Charles Young Dr. South/ 4726 MRL
|
| City |
LA |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE92886 |
Modulation of EMP2 in HEC1A cells |
|
