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Sample GSM2430946 Query DataSets for GSM2430946
Status Public on Jan 22, 2017
Title Aire KO Topo 1
Sample type RNA
 
Source name Thymus
Organism Mus musculus
Characteristics age: 6 Weeks Old
Sex: Female
strain: C57Bl/6
genotype: Aire-/-
cells: MECs
treatment: Topo
amplification technique: Ambion WT Expression Kit
separation method: Dissection / Trimming
Extracted molecule total RNA
Extraction protocol TriZol
Label Biotin
Label protocol Affymetrix GeneChip WT Terminal Labeling and Controls Kit
 
Hybridization protocol Ambion WT Expression Kit
Scan protocol Affymetrix GeneChip Scanner
Data processing The datasets was pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Dec 16, 2016
Last update date Jan 22, 2017
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL6246
Series (2)
GSE92509 Aire, guardian of immunological tolerance, binds to and activates super-enhancers [expression]
GSE92654 Aire, guardian of immunological tolerance, binds to and activates super-enhancers

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 43.305
10344622 235.7233
10344624 1399.2033
10344633 1503.4309
10344637 751.6613
10344653 58.1028
10344658 595.4846
10344674 36.8955
10344679 325.8354
10344707 1041.0452
10344713 228.0577
10344723 75.7416
10344725 259.7522
10344741 709.6142
10344743 82.0801
10344750 1589.7755
10344772 72.6506
10344789 478.0258
10344797 623.0335
10344799 627.504

Total number of rows: 24922

Table truncated, full table size 433 Kbytes.




Supplementary file Size Download File type/resource
GSM2430946_EA07068_326096_MOGENE-1_0-ST-V1_MECHI.AIRE.T1_1.CEL.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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