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Status
Public on Jan 22, 2017
Title
Aire KO Topo 1
Sample type
RNA
Source name
Thymus
Organism
Mus musculus
Characteristics
age: 6 Weeks Old Sex: Female strain: C57Bl/6 genotype: Aire-/- cells: MECs treatment: Topo amplification technique: Ambion WT Expression Kit separation method: Dissection / Trimming
Extracted molecule
total RNA
Extraction protocol
TriZol
Label
Biotin
Label protocol
Affymetrix GeneChip WT Terminal Labeling and Controls Kit
Hybridization protocol
Ambion WT Expression Kit
Scan protocol
Affymetrix GeneChip Scanner
Data processing
The datasets was pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Dec 16, 2016
Last update date
Jan 22, 2017
Contact name
CBDM Lab
E-mail(s)
cbdm@hms.harvard.edu
Phone
617-432-7747
Organization name
Harvard Medical School
Department
Microbiology and Immunobiology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02215
Country
USA
Platform ID
GPL6246
Series (2)
GSE92509
Aire, guardian of immunological tolerance, binds to and activates super-enhancers [expression]
GSE92654
Aire, guardian of immunological tolerance, binds to and activates super-enhancers
Data table header descriptions
ID_REF
VALUE
RMA normalized signal intensity
Data table
ID_REF
VALUE
10344620
43.305
10344622
235.7233
10344624
1399.2033
10344633
1503.4309
10344637
751.6613
10344653
58.1028
10344658
595.4846
10344674
36.8955
10344679
325.8354
10344707
1041.0452
10344713
228.0577
10344723
75.7416
10344725
259.7522
10344741
709.6142
10344743
82.0801
10344750
1589.7755
10344772
72.6506
10344789
478.0258
10344797
623.0335
10344799
627.504
Total number of rows: 24922 Table truncated, full table size 433 Kbytes .Supplementary file
Size
Download
File type/resource
GSM2430946_EA07068_326096_MOGENE-1_0-ST-V1_MECHI.AIRE.T1_1.CEL.gz
3.4 Mb
(ftp) (http)
CEL
Processed data included within Sample table