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Sample GSM2430945 Query DataSets for GSM2430945
Status Public on Jan 22, 2017
Title Aire KO Etopo 2
Sample type RNA
 
Source name Thymus
Organism Mus musculus
Characteristics age: 6 Weeks Old
Sex: Female
strain: C57Bl/6
genotype: Aire-/-
cells: MECs
treatment: Etopo
amplification technique: Ambion WT Expression Kit
separation method: Dissection / Trimming
Extracted molecule total RNA
Extraction protocol TriZol
Label Biotin
Label protocol Affymetrix GeneChip WT Terminal Labeling and Controls Kit
 
Hybridization protocol Ambion WT Expression Kit
Scan protocol Affymetrix GeneChip Scanner
Data processing The datasets was pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Dec 16, 2016
Last update date Jan 22, 2017
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL6246
Series (2)
GSE92509 Aire, guardian of immunological tolerance, binds to and activates super-enhancers [expression]
GSE92654 Aire, guardian of immunological tolerance, binds to and activates super-enhancers

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 32.4875
10344622 313.741
10344624 1395.6155
10344633 1653.7664
10344637 669.8895
10344653 47.4259
10344658 445.1013
10344674 29.3606
10344679 181.1129
10344707 908.3176
10344713 461.0864
10344723 137.5608
10344725 207.9013
10344741 1248.8344
10344743 70.7417
10344750 1531.8795
10344772 86.1011
10344789 486.7079
10344797 647.9938
10344799 575.5728

Total number of rows: 24922

Table truncated, full table size 433 Kbytes.




Supplementary file Size Download File type/resource
GSM2430945_EA07068_371484_MOGENE-1_0-ST-V1_MECHI.AIRE.ETOPO_2.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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