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Status
Public on Jan 22, 2017
Title
Aire KO Etopo 2
Sample type
RNA
Source name
Thymus
Organism
Mus musculus
Characteristics
age: 6 Weeks Old Sex: Female strain: C57Bl/6 genotype: Aire-/- cells: MECs treatment: Etopo amplification technique: Ambion WT Expression Kit separation method: Dissection / Trimming
Extracted molecule
total RNA
Extraction protocol
TriZol
Label
Biotin
Label protocol
Affymetrix GeneChip WT Terminal Labeling and Controls Kit
Hybridization protocol
Ambion WT Expression Kit
Scan protocol
Affymetrix GeneChip Scanner
Data processing
The datasets was pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Dec 16, 2016
Last update date
Jan 22, 2017
Contact name
CBDM Lab
E-mail(s)
cbdm@hms.harvard.edu
Phone
617-432-7747
Organization name
Harvard Medical School
Department
Microbiology and Immunobiology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02215
Country
USA
Platform ID
GPL6246
Series (2)
GSE92509
Aire, guardian of immunological tolerance, binds to and activates super-enhancers [expression]
GSE92654
Aire, guardian of immunological tolerance, binds to and activates super-enhancers
Data table header descriptions
ID_REF
VALUE
RMA normalized signal intensity
Data table
ID_REF
VALUE
10344620
32.4875
10344622
313.741
10344624
1395.6155
10344633
1653.7664
10344637
669.8895
10344653
47.4259
10344658
445.1013
10344674
29.3606
10344679
181.1129
10344707
908.3176
10344713
461.0864
10344723
137.5608
10344725
207.9013
10344741
1248.8344
10344743
70.7417
10344750
1531.8795
10344772
86.1011
10344789
486.7079
10344797
647.9938
10344799
575.5728
Total number of rows: 24922 Table truncated, full table size 433 Kbytes .Supplementary file
Size
Download
File type/resource
GSM2430945_EA07068_371484_MOGENE-1_0-ST-V1_MECHI.AIRE.ETOPO_2.CEL.gz
4.4 Mb
(ftp) (http)
CEL
Processed data included within Sample table