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Status
Public on Jan 22, 2017
Title
WT Etopo Topo 3
Sample type
RNA
Source name
Thymus
Organism
Mus musculus
Characteristics
age: 4 Weeks Old Sex: Female strain: C57Bl/6 genotype: Aire+/+ cells: MECs treatment: Etopo Topo amplification technique: Ambion WT Expression Kit separation method: Dissection / Trimming
Extracted molecule
total RNA
Extraction protocol
TriZol
Label
Biotin
Label protocol
Affymetrix GeneChip WT Terminal Labeling and Controls Kit
Hybridization protocol
Ambion WT Expression Kit
Scan protocol
Affymetrix GeneChip Scanner
Data processing
The datasets was pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Dec 16, 2016
Last update date
Jan 22, 2017
Contact name
CBDM Lab
E-mail(s)
cbdm@hms.harvard.edu
Phone
617-432-7747
Organization name
Harvard Medical School
Department
Microbiology and Immunobiology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02215
Country
USA
Platform ID
GPL6246
Series (2)
GSE92509
Aire, guardian of immunological tolerance, binds to and activates super-enhancers [expression]
GSE92654
Aire, guardian of immunological tolerance, binds to and activates super-enhancers
Data table header descriptions
ID_REF
VALUE
RMA normalized signal intensity
Data table
ID_REF
VALUE
10344620
40.9302
10344622
227.2429
10344624
1005.6611
10344633
1444.4108
10344637
472.6328
10344653
67.3649
10344658
409.9015
10344674
44.7671
10344679
356.4564
10344707
572.511
10344713
474.5801
10344723
116.7308
10344725
153.6423
10344741
510.2336
10344743
95.0056
10344750
719.7029
10344772
66.6031
10344789
425.9249
10344797
718.4257
10344799
397.7979
Total number of rows: 24922 Table truncated, full table size 433 Kbytes .Supplementary file
Size
Download
File type/resource
GSM2430943_EA07068_414681_MOGENE-1_0-ST-V1_MECHI.WT.TOET_4.CEL.gz
4.0 Mb
(ftp) (http)
CEL
Processed data included within Sample table