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Sample GSM2430943 Query DataSets for GSM2430943
Status Public on Jan 22, 2017
Title WT Etopo Topo 3
Sample type RNA
 
Source name Thymus
Organism Mus musculus
Characteristics age: 4 Weeks Old
Sex: Female
strain: C57Bl/6
genotype: Aire+/+
cells: MECs
treatment: Etopo Topo
amplification technique: Ambion WT Expression Kit
separation method: Dissection / Trimming
Extracted molecule total RNA
Extraction protocol TriZol
Label Biotin
Label protocol Affymetrix GeneChip WT Terminal Labeling and Controls Kit
 
Hybridization protocol Ambion WT Expression Kit
Scan protocol Affymetrix GeneChip Scanner
Data processing The datasets was pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Dec 16, 2016
Last update date Jan 22, 2017
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL6246
Series (2)
GSE92509 Aire, guardian of immunological tolerance, binds to and activates super-enhancers [expression]
GSE92654 Aire, guardian of immunological tolerance, binds to and activates super-enhancers

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 40.9302
10344622 227.2429
10344624 1005.6611
10344633 1444.4108
10344637 472.6328
10344653 67.3649
10344658 409.9015
10344674 44.7671
10344679 356.4564
10344707 572.511
10344713 474.5801
10344723 116.7308
10344725 153.6423
10344741 510.2336
10344743 95.0056
10344750 719.7029
10344772 66.6031
10344789 425.9249
10344797 718.4257
10344799 397.7979

Total number of rows: 24922

Table truncated, full table size 433 Kbytes.




Supplementary file Size Download File type/resource
GSM2430943_EA07068_414681_MOGENE-1_0-ST-V1_MECHI.WT.TOET_4.CEL.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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