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Status
Public on Jan 22, 2017
Title
WT Etopo 1
Sample type
RNA
Source name
Thymus
Organism
Mus musculus
Characteristics
age: 6 Weeks Old Sex: Female strain: C57Bl/6 genotype: Aire+/+ cells: MECs treatment: Etopo amplification technique: Ambion WT Expression Kit separation method: Dissection / Trimming
Extracted molecule
total RNA
Extraction protocol
TriZol
Label
Biotin
Label protocol
Affymetrix GeneChip WT Terminal Labeling and Controls Kit
Hybridization protocol
Ambion WT Expression Kit
Scan protocol
Affymetrix GeneChip Scanner
Data processing
The datasets was pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Dec 16, 2016
Last update date
Jan 22, 2017
Contact name
CBDM Lab
E-mail(s)
cbdm@hms.harvard.edu
Phone
617-432-7747
Organization name
Harvard Medical School
Department
Microbiology and Immunobiology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02215
Country
USA
Platform ID
GPL6246
Series (2)
GSE92509
Aire, guardian of immunological tolerance, binds to and activates super-enhancers [expression]
GSE92654
Aire, guardian of immunological tolerance, binds to and activates super-enhancers
Data table header descriptions
ID_REF
VALUE
RMA normalized signal intensity
Data table
ID_REF
VALUE
10344620
27.2919
10344622
380.7956
10344624
1432.0335
10344633
1379.1152
10344637
647.008
10344653
43.3843
10344658
499.9464
10344674
25.7407
10344679
246.788
10344707
714.9757
10344713
679.5998
10344723
98.9789
10344725
171.2088
10344741
1240.8598
10344743
87.985
10344750
1123.0128
10344772
65.0187
10344789
435.8238
10344797
624.6327
10344799
397.7027
Total number of rows: 24922 Table truncated, full table size 433 Kbytes .Supplementary file
Size
Download
File type/resource
GSM2430935_EA07068_371492_MOGENE-1_0-ST-V1_MECHI.WT.ETOPO_1.CEL.gz
4.6 Mb
(ftp) (http)
CEL
Processed data included within Sample table