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Sample GSM2430935 Query DataSets for GSM2430935
Status Public on Jan 22, 2017
Title WT Etopo 1
Sample type RNA
 
Source name Thymus
Organism Mus musculus
Characteristics age: 6 Weeks Old
Sex: Female
strain: C57Bl/6
genotype: Aire+/+
cells: MECs
treatment: Etopo
amplification technique: Ambion WT Expression Kit
separation method: Dissection / Trimming
Extracted molecule total RNA
Extraction protocol TriZol
Label Biotin
Label protocol Affymetrix GeneChip WT Terminal Labeling and Controls Kit
 
Hybridization protocol Ambion WT Expression Kit
Scan protocol Affymetrix GeneChip Scanner
Data processing The datasets was pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Dec 16, 2016
Last update date Jan 22, 2017
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL6246
Series (2)
GSE92509 Aire, guardian of immunological tolerance, binds to and activates super-enhancers [expression]
GSE92654 Aire, guardian of immunological tolerance, binds to and activates super-enhancers

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 27.2919
10344622 380.7956
10344624 1432.0335
10344633 1379.1152
10344637 647.008
10344653 43.3843
10344658 499.9464
10344674 25.7407
10344679 246.788
10344707 714.9757
10344713 679.5998
10344723 98.9789
10344725 171.2088
10344741 1240.8598
10344743 87.985
10344750 1123.0128
10344772 65.0187
10344789 435.8238
10344797 624.6327
10344799 397.7027

Total number of rows: 24922

Table truncated, full table size 433 Kbytes.




Supplementary file Size Download File type/resource
GSM2430935_EA07068_371492_MOGENE-1_0-ST-V1_MECHI.WT.ETOPO_1.CEL.gz 4.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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