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Status
Public on Jan 22, 2017
Title
Aire KO DMSO 2
Sample type
RNA
Source name
Thymus
Organism
Mus musculus
Characteristics
age: 6 Weeks Old Sex: Female strain: C57Bl/6 genotype: Aire-/- cells: MECs treatment: DMSO amplification technique: Ambion WT Expression Kit separation method: Dissection / Trimming
Extracted molecule
total RNA
Extraction protocol
TriZol
Label
Biotin
Label protocol
Affymetrix GeneChip WT Terminal Labeling and Controls Kit
Hybridization protocol
Ambion WT Expression Kit
Scan protocol
Affymetrix GeneChip Scanner
Data processing
The datasets was pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Dec 16, 2016
Last update date
Jan 22, 2017
Contact name
CBDM Lab
E-mail(s)
cbdm@hms.harvard.edu
Phone
617-432-7747
Organization name
Harvard Medical School
Department
Microbiology and Immunobiology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02215
Country
USA
Platform ID
GPL6246
Series (2)
GSE92509
Aire, guardian of immunological tolerance, binds to and activates super-enhancers [expression]
GSE92654
Aire, guardian of immunological tolerance, binds to and activates super-enhancers
Data table header descriptions
ID_REF
VALUE
RMA normalized signal intensity
Data table
ID_REF
VALUE
10344620
34.4808
10344622
358.9242
10344624
1505.186
10344633
1799.6378
10344637
703.9781
10344653
44.9681
10344658
425.0057
10344674
31.2631
10344679
180.3753
10344707
841.5369
10344713
495.7478
10344723
96.2674
10344725
196.2788
10344741
933.2522
10344743
72.8835
10344750
1250.2816
10344772
78.8373
10344789
385.711
10344797
557.4759
10344799
490.5143
Total number of rows: 24922 Table truncated, full table size 433 Kbytes .Supplementary file
Size
Download
File type/resource
GSM2430933_EA07068_371487_MOGENE-1_0-ST-V1_MECHI.AIRE.DMSO_3.CEL.gz
4.5 Mb
(ftp) (http)
CEL
Processed data included within Sample table