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Sample GSM2430933 Query DataSets for GSM2430933
Status Public on Jan 22, 2017
Title Aire KO DMSO 2
Sample type RNA
 
Source name Thymus
Organism Mus musculus
Characteristics age: 6 Weeks Old
Sex: Female
strain: C57Bl/6
genotype: Aire-/-
cells: MECs
treatment: DMSO
amplification technique: Ambion WT Expression Kit
separation method: Dissection / Trimming
Extracted molecule total RNA
Extraction protocol TriZol
Label Biotin
Label protocol Affymetrix GeneChip WT Terminal Labeling and Controls Kit
 
Hybridization protocol Ambion WT Expression Kit
Scan protocol Affymetrix GeneChip Scanner
Data processing The datasets was pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Dec 16, 2016
Last update date Jan 22, 2017
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL6246
Series (2)
GSE92509 Aire, guardian of immunological tolerance, binds to and activates super-enhancers [expression]
GSE92654 Aire, guardian of immunological tolerance, binds to and activates super-enhancers

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 34.4808
10344622 358.9242
10344624 1505.186
10344633 1799.6378
10344637 703.9781
10344653 44.9681
10344658 425.0057
10344674 31.2631
10344679 180.3753
10344707 841.5369
10344713 495.7478
10344723 96.2674
10344725 196.2788
10344741 933.2522
10344743 72.8835
10344750 1250.2816
10344772 78.8373
10344789 385.711
10344797 557.4759
10344799 490.5143

Total number of rows: 24922

Table truncated, full table size 433 Kbytes.




Supplementary file Size Download File type/resource
GSM2430933_EA07068_371487_MOGENE-1_0-ST-V1_MECHI.AIRE.DMSO_3.CEL.gz 4.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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