|
| Status |
Public on Jan 13, 2017 |
| Title |
Mouse 14.5 Mutant Alk5 D_Mandible _Rep2 |
| Sample type |
RNA |
| |
|
| Source name |
mouse embyo stage E14.5
|
| Organism |
Mus musculus |
| Characteristics |
background strain: C57B6
tissue: Mandible
tissue compartment: Distal bone
genotype: Alk5 mutant
Stage: E14.5
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from tissues using RNeasy kit (Qiagen Valenica, CA)
|
| Label |
biotin
|
| Label protocol |
Starting from 5 µg total RNA, labeled cRNA was prepared using the Qiagen Microarray cDNA Synthesis, Microarray RNA Target Synthesis (T7) and Microarray Target Purification Kit, according to the manufacturer's instructions
|
| |
|
| Hybridization protocol |
Following fragmentation, 12 µg of cRNA was hybridized for 16hr on Affymetrix Mouse 430_2 (gene version) as recommended by the manufacturer
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
|
| Description |
Gene expression
|
| Data processing |
The probe level data was summarized to gene level and was analyzed with PGS 6.6 . RMA (Robust Multi array average) normalization was used
|
| |
|
| Submission date |
Dec 14, 2016 |
| Last update date |
Feb 28, 2019 |
| Contact name |
Michael Kesling |
| E-mail(s) |
michael.kesling@alumni.stanford.edu
|
| Phone |
415-325-4073
|
| Organization name |
University of Southern California
|
| Street address |
2250 Alcazar Street
|
| City |
Los Angeles |
| ZIP/Postal code |
90033 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE92420 |
Integrated functional genomics and craniofacial morphogenesis within the FaceBase Consortium: Alk5 mutant |
| GSE93333 |
Integrated functional genomics and craniofacial morphogenesis within the FaceBase Consortium: Alk5 and TGFBR2 mutants |
|
